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Lack of end-product control of nitrite reductase level in pea roots

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Biologia Plantarum

Abstract

The effects of various ammonium salts and amino acids on nitrite reductase (NIR) induction in isolated pea roots cultured in media containing nitrate or nitrite and either exogenous sucrose or no sugar were investigated. Thg aim of these investigations was to determine if the NIR level is subject to end-product control. The results showed that even though some ammonium salts and casamino acids can depress NIR level under certain conditions this inhibition cannot be interpreted in terms of direct end-product inhibition of NIR synthesis because their effects were dependent on the character (anion) and toxicity of the respective ammonium salt, on the presence of exogenous sucrose in the induction medium, and on the inducer of NIR. NH4HCO3 inhibited NIR induction at those concentrations which were toxic to the roots, ammonium phosphates hampered NIR induction only in roots exposed to nitrite in media containing sucrose, while casamino acids slightly depressed NIR induction only in roots exposed to nitrate and exogenous sucrose. The results further show that the basal (noninduced) NIR level changes little even under strongly toxic conditions.

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References

  • Ferguson, A. R.: The nitrogen metabolism ofSpirodella oligorhiza. II. Control of the enzymes of nitrate assimilation. -Planta88: 353–363, 1969.

    Article  CAS  Google Scholar 

  • Heimer, Y. M., Riklis, E.: Posttranscriptional control of nitrate reductase of cultured tobacco cells by amino acids. - Plant Physiol.64: 663–664, 1979.

    PubMed  CAS  Google Scholar 

  • Ingle, J., Joy, K. W., Hageman, R. H.: The regulation of activity of the enzymes involved in the assimilation of nitrate by higher plants. - Biochem. J.100: 577–588, 1966.

    PubMed  CAS  Google Scholar 

  • Joy, K. W.: Nitrogen metabolism ofLemna minor. II. Enzymes of nitrate assimilation and some aspects of their regulation. -Plant Physiol.44: 849–853, 1969.

    PubMed  CAS  Google Scholar 

  • Kirkby, E. A., Mengel, K.: Ionic balance in different tissues of the tomato plant in relation to nitrate, urea or ammonium nutrition. - Plant Physiol.42: 6–14, 1967.

    PubMed  CAS  Google Scholar 

  • Morris, I., Syrett, P. J.: Cyanate inhibition of nitrate reductase. - Biochim. biophys. Acta77: 649–650, 1963.

    Article  CAS  Google Scholar 

  • Raven, J. A., Smith, F. A.: Nitrogen assimilation and transport in vascular land plants in relation to intracellular pH regulation. - New Phytol.76: 415–432, 1976.

    Article  CAS  Google Scholar 

  • Sahulka, J.: The regulation of nitrate reductase, nitrite reductase and glutamate dehydrogenase in excised pea roots by some exogenous amino acids. - Biol. Plant.14: 308–311, 1972.

    Article  CAS  Google Scholar 

  • Sahulka, J.: The regulation of glutamate dehydrogenase, nitrite reductase, and nitrate reductase in excised pea roots by nitrite.- Biol. Plant.15: 298–301, 1973.

    CAS  Google Scholar 

  • Sahulka, J.: The effect of some ammonium salts on nitrate reductase level, onin vivo nitrate reduction and on nitrate content in excisedPisum sativum roots. - Biol. Plant.19: 113–128, 1977.

    CAS  Google Scholar 

  • Sahulka, J.: The effect of some carbohydrates on nitrite reductase level in isolated pea roots. - Plant Sci. Lett.23: 169–175, 1981.

    Article  CAS  Google Scholar 

  • Sahulka, J., Lisá, L.: Regulation of glutamine synthetase level in isolated pea roots. I. Differential effects of ammonium salts in sugar-supplied roots. - Biochem. Physiol. Pflanzen174: 646–652, 1979a.

    CAS  Google Scholar 

  • Sahulka, J., Lisá, L.: Regulation of glutamine synthetase level in isolated pea roots. II. The effect of exogenously supplied acids and bases in sugar-supplied and sugar-starved roots. - Biochem. Physiol. Pflanzen174: 653–659, 1979b.

    CAS  Google Scholar 

  • Sahulka, J., Lisá, L.: Effect of some disaccharides, hexoses and pentoses on nitrate reductase, glutamine synthetase and glutamate dehydrogenase in excised pea roots. - Physiol. Plant.50: 32–36, 1980.

    Article  CAS  Google Scholar 

  • Sahulka, J., Lisá, L.: Differential effects of exogenous L-glutamine, L-glutamie acid, Na glutamate and casamino acids on glutamine synthetase and nitrate reductase in isolated pea roots cultured with or without sucrose. - Can. J. Bot.59: 1121–1127, 1981.

    CAS  Google Scholar 

  • Smith, F. A., Raven, J. A.: Intracellular pH and its regulation. - Annu. Rev. Plant Physiol.30: 289–311, 1979.

    Article  CAS  Google Scholar 

  • Stewart, G. R.: The regulation of nitrite reductase level inLemna minor L. - J. exp. Bot.23: 171–183, 1972.

    Article  CAS  Google Scholar 

  • Vega, J. M., Herrera, J., Relimpio, A. M., Aparicio, P. J.: NADH-nitrate réductaso deChlorella: nouvelle contribution à l’étude de ces propriétés. - Physiol. vég.10: 637–652, 1972.

    CAS  Google Scholar 

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  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Ke dvoru 16/15, 166 30, Praha 6, Czechoslovakia

    J. Sahulka

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  1. J. Sahulka
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Sahulka, J. Lack of end-product control of nitrite reductase level in pea roots. Biol Plant 24, 461–467 (1982). https://doi.org/10.1007/BF02880445

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  • DOI: https://doi.org/10.1007/BF02880445

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