Abstract
Crown galls induced onArabidopsis thaliana plants by octopine or nopaline strains ofAgrobacterium tumefaciens were grownin vitro on different media. Dark growth of all tumor tissues was strictly hormone-dependent. In contrast, hormonal autonomy was observed in the light where crown gall calli readily differentiated into teratomas and (sometimes fertile) plants. Differentiating tissues always grew more vigorously than subtended calli. The growth of transformed calli was stimulated by vitamins and partly inhibited by growth regulators in concentrations used for the maintenance of untransformed calli. Crown gall calli, teratomas and sometimes regenerated plants were shown to express lysopine or nopaline dehydrogenase activities.
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Abbreviations
- 2,4-D:
-
2,4-dichlorphenoxyacetic acid
References
Aebts, M., Jacobs, M., Hernalsteehs, J. P., Van Montagu, M.,Schell, J.: Induction andin vitro culture ofArabidopsis thaliana crown galls. - Plant Sci. Lett.17: 43–50, 1979.
Chilton, M. D., Drummond, M. H., Merlo, D. J., Sciaky, D., Montoya, M. H., Gordon, M. P., Nester, E. W.: Stable incorporation of plasmid DNA into higher plant cells: The molecular basis of crown gall tumorigenesis. - Cell11: 263–271, 1977.
Gamborg, O. L., Miller, R. A., Ojima, K.: Nutrient requirement of suspension culture of soybean root cells. - Exp. Cell Res.60: 151–168, 1968.
Gleba, Y. Y., Hoffmann, F.; Hybrid cell lines ofArabidopsis thaliana andBrassica campestris: No evidence for specific chromosome elimination. - Mol. gen. Genet.165: 257–264, 1978.
Langley, R. A., Kado, C. I.: Studies ofAgrobacterium tumefaciens. Conditions for mutagenesis by N-methyl-N’-nitro-N-nitrosoquanidine and relationship ofA. tumefaciens mutants to crown gall tumor induction. - Mutation Res.14: 277–286, 1972.
Leemans, J., Shaw, C., Deblaere, R., De Greve, H., Hernalsteens, J. P., Maes, M., Van Montagu, M.,Schell, J.: Site-specific mutagenesis ofAgrobacterium Ti plasmids and transfer of genes to plant cells. - J. mol. appl. Genet.1: 149–164, 1981.
Matzke, A. J. M., Chilton, M. D.: Site specific insertion of genes into the T-DNA ofAgrobacterium tumefaciens tumor-inducing plasmid: an approach to genetic engineering in higher plant cells. - J. mol. appl. Genet.1: 39–49, 1981.
Negrutiu, I., Beefting, F., Jacobs, M.:Arabidopsis thaliana as a model system in somatic cell genetics I. Cell and tissue culture. - Plant Sci. Lett.5: 293–304, 1975.
Otten, L., Schilperoort, R. A.: A rapid micro scale method for the detection of lysopine and nopaline dehydrogenase activities. - Biochim. biophys. Acta527: 497–500, 1978.
Willmitzeb, L., Simons, G., Schell, J.: The TL-DNA in octopine crown gall tumors codes for seven well-defined polyadenylated transcripts. - EMBO J.1: 139–146, 1982.
Xuan, L. T., Menczel, L.: Improved protoplast culture and plant regeneration from protoplast-derived callus inArabidopsis thaliana. - Z. Pflanzenphysiol.96: 77–80, 1980.
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Ondřej, M., Pavingerová, D., Našinec, V. et al. Growth requirements ofArabidopsis thaliana crown galls. Biol Plant 26, 5–10 (1984). https://doi.org/10.1007/BF02880416
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DOI: https://doi.org/10.1007/BF02880416