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Formation of biologically active substances by rhizosphere bacteria and their effect on plant growth

Folia Microbiologica volume 15, pages 468–478 (1970)Cite this article

Abstract

Nine out of seventeen strains of bacteria with a pronounced effect on seed germination and on seedling growth, isolated from root surfaces and rhizosphere soil of maize, were selected for a study on the formation of biologically active substances. β-Indole acetic acid (45–72 μg/1.000 ml) was produced by four strains, gibberelline-like substances (1.0–60.0 μg/1.000ml) by all strains, biotin and pantothenic acid by the majority of strains and nicotinic acid by five strains. Amino acids were formed by all strains but in low amounts. Four strains produced growth inhibitors. The highest amounts of biologically active substances were found in cultures ofPseudomonas fluorescens andBacillus brevis. The various cultures ofPseudomonas fluorescens differed in their capability to produce biologically active substances. The majority of bacterial cultures or their supernatants significantly stimulated the germination of seeds and some of them significantly affected the growth of plants. Inoculation of maize seeds with strainsPseudomonas fluorescens andChromobacterium violaceum significantly increased the yield of dry matter of plants.

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References

  • Batson, H. C.:An Introduction to Statistics in the Medical Sciences. Burgess Publ. Co., Minneseta, 1956.

    Google Scholar 

  • Bojarkin, A. N., Dimitrieva, M. J.:Biological gibberellin tests. (In Russian). Fyziologiya rastenij 6: 741, 1959.

    Google Scholar 

  • Borris, H.:Biologische und chemische Methoden zum Nachweis der pflanzlichen Streckungswuchsstoffe (Auxine). In A. N. Beloserski, N. Y. Proskurjakov: Praktikum der Biochemie der Pflanzen, p. 389–405, Berlin, 1956.

  • Bowen, G. D., Rovira, A. D.:The effect of microorganisms on plant growth. I. Development of root hairs in sand and agar. Plant and Soil 15: 168, 1961.

    Google Scholar 

  • Brian, P. W., Hemming, H. G., Lowe, D.:Comparative potency of nine gibberellins. Annals Botany, N. S. 28: 369, 1964.

    CAS  Google Scholar 

  • Brown, M. E., Burlingham, S. K.:Production of plant growth substances by Azotobacter chroococcum. J. gen. Microbiol. 53: 135, 1968.

    PubMed  CAS  Google Scholar 

  • Brown, M. E., Burlingham, S. K., Jackson, R. M.:Studies of Azotobacter species in soil. III. Effect of artificial inoculation on crop yields. Plant and Soil 20: 194, 1964.

    Article  Google Scholar 

  • Chalvignac, M. A., Falcou, Y.:Bactérisation des graines et physiologie de la plante. II. Inoculation du maĩs par Ps. fluorescens. Ann Inst. Pasteur (Suppl.) 111: 49, 1966.

    Google Scholar 

  • Dörr, R.:Die Aufnahme von Brucin durch Pflanzenwurzeln aus Nährlösung. Z. Pflanzenernähr. Bodenk. 116: 96, 1967.

    Article  Google Scholar 

  • Holding, A. Y.:The properties and classification of the predominant gram negative bacteria occurring in soil. J. appl. Bacteriol. 23: 516, 1960.

    Google Scholar 

  • Jackson, R. M., Brown, M. E., Burlingham, S. K.:Similar effect on tomato plants of Azotobacter inoculation and application of gibberellins. Nature 203: 851, 1964.

    Article  CAS  Google Scholar 

  • Katznelson, H.:Nature and importance of the rhizosphere. In Ecology of Soil Born Plant Pathogens. Univ. Calif. Press. Berkeley, 1965.

    Google Scholar 

  • Katznelson, H., Cole, C. E.:Production of gibberellin like substances by bacteria and actinomycetes. Can. J. Microbiol. 11: 733, 1965.

    PubMed  CAS  Google Scholar 

  • Katznelson, H., Sirois, J. C.:Auxin production by species of Artrobacter. Nature 191: 1323, 1961.

    PubMed  Article  CAS  Google Scholar 

  • Kerr, A.:A study of tomato root surface organisms antagonistic to Verticillium albo-atrum. Trans. Brit. Mycol. Soc. 44: 365, 1961.

    Article  Google Scholar 

  • Kononova, M. M., Diakonova, K. T.:Soil organic matter and problems in nutrition of plants. (In Russian). Pochvovedenie 3: 1, 1960.

    Google Scholar 

  • Krasilnikov, N. A.:The role of microorganisms in plant life. In Recent progress in Microbiology. University of Toronto Press, 1963.

  • Macura, J.:Seed and soil bacteria in relation to the rhizosphere effect. Fol. biol. (Praha) 4: 274, 1958.

    Google Scholar 

  • Macura, J.:Physiological studies of rhizosphere bacteria. Int. Symposium Ecology of Soil Bacteria, p. 379, Liverpool, 1968.

  • Miettinen, J. K.:Assimilation of amino acids in higher plants. Symposia Soc. exptl. Biol. 13: 210, 1959.

    Google Scholar 

  • Miller, R. H., Schmidt, E. L.:Uptake and assimilation of amino acids supplied to the sterile soil: root environment of the bean plant (Phaseolus vulgaris). Soil Science 100: 323, 1965.

    Article  CAS  Google Scholar 

  • Nordstadt, F. A., MacCalla, T. M.:Phytotoxic substance from a species of Penicillium. Science 140: 310, 1963.

    Article  Google Scholar 

  • Odinceva, E. N.:Microbiological methods of determinations of vitamins. (In Russian) Publ. House Acad. Sci. USSR Moscow, 1959.

    Google Scholar 

  • Overbeck, V., Racusen, D. W., Tagami, M., Huges, W. Y.:Simultaneous analysis of auxins and gibberellin. Plant Physiol. 32: XXXII (Supl.) 1957.

    Google Scholar 

  • Pantos, G.:The vitamin synthetising capacity of some dominant strains of bacteria in the rhizosphere of wheat and maize. Agrokem. Talajt. 10: 511, 1961.

    CAS  Google Scholar 

  • Rouatt, J. W., Katznelson, H.:The comparative growth of bacterial isolates from rhizosphere and nonrhizosphere soils. Can. J. Microbiol. 3: 271, 1957.

    Article  Google Scholar 

  • Rovira, A. D.:Interactions between plant roots and soil microorganisms. Ann. Rev. Microbiol. 19: 241, 1965.

    Article  CAS  Google Scholar 

  • Rybalkina, A. V., Kononenko, Je. V.:Microflora of decomposing plant residues. (In Russian) Pochvovedenie 5: 21, 1959.

    Google Scholar 

  • Scheffer, F., Kickuth, R., Aldag, R.:Einwirkung von d-Leucin auf Helianthus annus Z. Naturwiss. 54: 144, 1967.

    CAS  Google Scholar 

  • Sequeria, L., Williams, P. H.:Synthesis of indole acetic acid by Pseudomonas solanacearum. Phytopath. 54: 1240, 1964.

    Google Scholar 

  • Smith, J.:Chromatographic and electrophoric techniques. W. Heinemann, London, 1960.

    Google Scholar 

  • Seear, J., Bradfute, O. E., McLaren, A. D.:Uptake of proteins by plant roots. Physiol. Plantarum 21: 979, 1968.

    Article  CAS  Google Scholar 

  • Sobieszezanski, J.:Role of microorganisms in the life of cultivated plants. I. Quantitative and qualitative changes in the microflora of the rhizosphere of rye and winter wetch during the vegetation period. Acta microbiol. Polon. 14: 161, 1965.

    Google Scholar 

  • Starkey, R. L.:Interrelations between microorganisms and plant roots in the rhizosphere. Bacteriol. Rev. 22: 154, 1958.

    PubMed  CAS  Google Scholar 

  • Taylor, C. B.:The nutritional requirements of the predominant flora of the soil. Proc. Soc. Appl. Bact. 14: 101, 1951.

    Google Scholar 

  • Vágnerová, K., Macura, J., Čatská, V.:Rhizosphere microflora of wheat. I. Composition and properties of bacterial flora during the first stages of growth. Fol. microbiol. 5: 298, 1960.

    Google Scholar 

  • Vágnerová, K., Vančura, V.:Production and utilization of amino acids by various species of rhizosphere bacteria. Fol. microbiol. 7: 55, 1962.

    Google Scholar 

  • Vágnerová, K., Vančura, V., Lasík, J.:The development of microorganisms of the root surface, rhizosphere and free soil in medium with root exudates. (In Czech) Rostlinná výroba (XXXVI) 9: 687, 1963.

    Google Scholar 

  • Vančura, V.:Detection of gibberellic acid in Azotobacter cultures. Nature 192: 88, 1961.

    PubMed  Article  Google Scholar 

  • Vančura, V., Macura, J.:Indole derivatives in Azotobacter cultures. Fol. microbiol. 5: 293, 1960.

    Article  Google Scholar 

  • Winter, A. G.:New physiological and biological aspects in the interrelationships between higher plants. Symposia Soc. Exptl. Biol. 15: 229, 1961.

    CAS  Google Scholar 

  • Woltz, S. S.:Growth modifying and antimetabolite effects of amino acids on Chrysanthenum. Plant Physiol. 38: 93, 1963.

    PubMed  CAS  Google Scholar 

  • Zarnescu, A., Nita, L.:The action of the metabolic products of microorganisms on higher plants. VIIIth International Congress of Soil Sci., Bucharest, Abstracts of papers 3: 106, 1964.

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Author notes

  1. A. Hussain

    Present address: Soil Science Department, West Pakistan Agricultural University, Lyallpur, Pakistan

Affiliations

  1. Department of Soil Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    A. Hussain & V. Vančura

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  1. A. Hussain
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  2. V. Vančura
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Hussain, A., Vančura, V. Formation of biologically active substances by rhizosphere bacteria and their effect on plant growth. Folia Microbiol 15, 468–478 (1970). https://doi.org/10.1007/BF02880191

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  • DOI: https://doi.org/10.1007/BF02880191

Keywords

  • Root Surface
  • Rhizosphere Soil
  • Bacillus Megaterium
  • Maize Seed
  • Sterile Soil