Skip to main content
SpringerLink
Log in

Tissue engineering and peripheral nerve regeneration (III)

—Sciatic nerve regeneration with PDLLA nerve guide

  • Published:
Science in China Series B: Chemistry Aims and scope Submit manuscript

Abstract

The biodegradation rate and biocompatibility of poly (d, / -lactide) (PDLLA)in vivo were evaluated. The aim of this study was to establish a nerve guide constructed by the PDLLA with 3-D microenvironment and to repair a 10 mm of sciatic nerve gap in rats. The process of the nerve regeneration was investigated by histological assessment, electrophysiological examination, and determination of wet weight recovery rate of the gastrocnemius muscle. After 3 weeks, the nerve guide had changed from a transparent to an opaque status. The conduit was degraded and absorbed partly and had lost their strength with breakage at the 9th week of postoperation. At the conclusion of 12 weeks, proximal and distal end of nerves were anastomosed by nerve regeneration and the conduit vanished completely. The results suggest that PDLLA conduits may serve for peripheral nerve regeneration and PDLLA is a sort of hopeful candidate for tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wang, S. G., Hou, J. W., Bei, J. Z., Tissue engineering and repair of peripheral nerve, High Technology Letters, 1999, 9(2): 60–62.

    Google Scholar 

  2. Doolabh, V. B., Hertl, M. C., Mackinnon, S. E., The role of conduits in nerve repair: A review, Rev. Neurosci., 1996, 7: 47–48.

    CAS  Google Scholar 

  3. Dunnen, W., Lei, B., Schakenread J. M. et al., Long-term evaluation of nerve regeneration in a biodegradable nerve guide, Microsurgery, 1993, 14: 508–515.

    Article  Google Scholar 

  4. Suzuki, K., Shizuya, O., Kitahara, K. et al., Evaluation of nerve ending regeneration by PGA-collagen terminal, Artificial Organs (Jap.), 1998, 27(2): 490–494.

    Google Scholar 

  5. Meek, M. F., DenDunnen, W. F., Schakenread, J. M. et al., Peripheral nerve regeneration and functional recovery after reconstruction with a thin-walled biodegradable poly(D, L-lactide-ε-caprolactone) nerve guide, Cells Mater., 1997, 7(1): 53–62.

    CAS  Google Scholar 

  6. Perego, G. D., Cella, G. D., Aldini, N. N. et al., Preparation of a new nerve guide from a poly(l-lactide-co-caprolactone), Biomaterials, 1994, 15: 189–193.

    Article  CAS  Google Scholar 

  7. Dunnen den, W. F. A., Lei van der, B., Schakenread, J. M. et al., Biological performance of a degradable poly(lactic acid-ε-caprolactone) nerve guide: Influence of tube dimension, J. Biomed. Mater. Res., 1995, 29: 757–766.

    Article  Google Scholar 

  8. Evans, G. R. D., Brandt, K., Widmer, M. S. et al.,In vivo evaluation of poly(X-lactic acid) porous conduits for peripheral nerve regeneration, Biomaterials, 1999, 20: 1109–1115.

    Article  CAS  Google Scholar 

  9. Rodriguez, F. J., Gomez, G., Perego, G. et al., Highly permeable polylactide-caprolactone nerve guide enhance peripheral nerve regeneration through long gaps, Biomaterials, 1999, 20: 1489–1500.

    Article  CAS  Google Scholar 

  10. Frazza, E. J., Schmitt, E. E., Anew absorbable suture, J. Biomed. Mater. Symp., 1971, 1: 43–58.

    Article  Google Scholar 

  11. Mikos, A. G., Thorsen, A. J., Czerwonka, L. A. et al., Preparation and characterization of poly(L-lactic acid) forms, Polymer, 1994, 35(5): 1068–1077.

    Article  CAS  Google Scholar 

  12. Lu, Z., Bei, J. Z., Wang, S. G., A method for the preparation of polymeric nanocapsules without stabilizer, Journal of Controlled Release, 1999, 61: 107–112.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, 100080, Beijing, China

    Shenguo Wang & Jianzhong Bei

  2. People’s Hospital of Henan Province, 450003, Zhengzhou, China

    Jianwei Hou & Yongqiang Zhao

Authors
  1. Shenguo Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jianwei Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianzhong Bei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yongqiang Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shenguo Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, S., Hou, J., Bei, J. et al. Tissue engineering and peripheral nerve regeneration (III). Sc. China Ser. B-Chem. 44, 419–426 (2001). https://doi.org/10.1007/BF02879817

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02879817

Keywords

Search

Navigation