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Thermostability of photosynthesis in two new chlorophyllb-less rice mutants

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Abstract

Leaves of the two new chlorophyllb-less rice mutants VG28-1, VG30-5 and the wild type rice cv. Zhonghua 11 were subjected to temperatures 28, 36, 40, 44 and 48°C in the dark for 30 min or gradually elevated temperature from 30°C to 80°C at 0.5°C/min. The thermostability of photosynthetic apparatus was estimated by the changes in chlorophyll fluorescence parameters, photosynthetic rate and pigment content, chloroplast ultrastructure and tissue location of H2O2 accumulation. There were different patterns of Fo-temperature curves between the Chlb-less mutants and the wild type plant, and the temperature of Fo rising threshold was shifted 3°C lower in the Chlb-less mutants (48°C) than in the wild type (51°C). At temperature up to about 45°C, chloroplasts were swollen and thylakoid grana became misty accompanied with the complete loss of photosynthetic oxygen evolution in the two Chlb-less mutants, but chloroplast ultrastructure in the wild type showed no obvious alteration. After 55°C exposure, the disordered thylakoid and significant H2O2 accumulation in leaves were found in the two Chlb-less mutants, whereas in the wild type plant, less H2O2 was accumulated and the swollen thylakoid still maintained a certain extent of stacking. A large extent of the changes in qP, NPQ and Fv/Fm was consistent with the Pn decreasing rate in the Chlb-less mutants during high temperature treatment as compared with the wild type. The results indicated that the Chlb-less mutants showed a tendency for higher thermosensitivity, and loss of Chlb in LHC II could lead to less thermostability of PSII structure and function. Heat damage to photosynthetic apparatus might be partially attributed to the internal oxidative stress produced at severely high temperature.

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References

  1. Georgieva, K., Yordanov, I., Temperature dependence of chlorophyll fluorescence parameters of pea seedlings, J. Plant Physiol., 1993, 142: 151–155.

    CAS  Google Scholar 

  2. Havaux, M., Characterization of thermal damage to the photosynthetic electron transport system in potato leaves, Plant Sci., 1993, 94: 19–33.

    Article  CAS  Google Scholar 

  3. Knaetzel, J., Simpson, D., Expression and organization of antenna proteins in the light- and temperature-sensitive barley mutant chlarina-104, Planta., 1991, 185: 111–123.

    Google Scholar 

  4. Bakhov, N. G., Sabat, S. C., Mohanty, P., Analysis of chlorophyll a fluorescence changes in weak light in heat treatedAmaranthus chloroplasts, Photosynth. Res., 1990, 23: 81–87.

    Article  Google Scholar 

  5. Schreiber, U., Berry, J. A., Heat-induced changes of chlorophyll fluorescence in intact leaves correlated with damage of the photosynthetic apparatus, Planta., 1977, 136: 233–238.

    Article  CAS  Google Scholar 

  6. Singh, A. K., Singhal, G. S., Specific degradation of D1 protein during exposure of thylakoid membranes to high temperature in dark, Photosynthetica., 1999, 36: 433–440.

    Article  CAS  Google Scholar 

  7. Yang, C. M., Osterman, J. C., Markwell, J., Temperature sensitivity as a general phenomenon in a collection of chlorophyll-deficient mutants of sweetclover (Melilotus alba), Biochem. Gen., 1990, 28: 31–40.

    Article  CAS  Google Scholar 

  8. Havaux, M., Tardy, F., Thermostability and photostability of photosystem II in leaves of the chlorina-f2 barley mutant deficient in light-harvesting chlorophylla/b protein complex, Plant Physiol., 1997, 113: 913–923.

    PubMed  CAS  Google Scholar 

  9. Wang, J., Lin, L., Wan, X. S. et al., Generation and molecular analysis of a population of transgenic rice plants carrying Ds element, Acta Phytophysiol. Sin., 2000, 26: 501–506.

    CAS  Google Scholar 

  10. Lin, Z. F., Peng, C. L., Lin, G. Z. et al., Photosynthetic characteristics of two new chlorophyll b-less rice mutants, Photosynthetica, 2003, 41: 61–67.

    Article  CAS  Google Scholar 

  11. Lin, Z. F., Lin, G. Z., Peng, C. L. et al., Alteration of chlorophyll-protein complex components and distribution of excitation energy between two photosystems in two new rice chlorophyll b-less mutants, Photosynthetica, 2003, 41: 589–595.

    Article  CAS  Google Scholar 

  12. Arnon, D. L., Copper enzymes in isolated chloroplast: Polyphenoloxidase inBeta vulgaris, Plant Physiol., 1949, 24: 1–15.

    PubMed  CAS  Google Scholar 

  13. Maxwell, K., Johnson, G. N., Chlorophyll fluorescence-a practical guide, J. Exp. Bot., 2000, 345: 659–668.

    Article  Google Scholar 

  14. Laisk, A., Oja, V., Rasulov, B. et al., Quantum yields and rate constants of photochemical and nonphotochemical excitation quenching, Plant Physiol., 1997, 115: 803–813.

    PubMed  CAS  Google Scholar 

  15. van Kooten, O., Snel, J. F. H., The use of chlorophyll fluorescence nomenclature in plant stress physiology, Photosynth. Res., 1990, 25: 147–150.

    Article  Google Scholar 

  16. Ganesan, V., Thomas, G., Salicylic acid response in rice: Influence of salicylic acid on H2O2 accumulation and oxidative stress, Plant Sci., 2001, 160: 1095–1106.

    Article  PubMed  CAS  Google Scholar 

  17. Gounaris, K., Brain, A. P. R., Quinn, P. J. et al., Structural reorganization of chloroplast thylakoid membranes in response to heat stress, Biochim. Biophys. Acta., 1984, 766: 198–208.

    Article  CAS  Google Scholar 

  18. Tan, X. X., Xu, D. Q., Shen, Y. K., Both spillover and light absorption cross-section changes are involved in the regulation of excitation energy distribution between the two photosystems during state transitions in wheat leaf, Photosynth. Res., 1998, 56: 95–102.

    CAS  Google Scholar 

  19. Vani, B., Saradhi, P. P., Mohanty, P., Alteration in chloroplast structure and thylakoid membrane composition due toin vivo heat treatment of rice seedlings: Correlation with the functional changes, J. Plant Physiol., 2001, 158: 583–592.

    Article  CAS  Google Scholar 

  20. Georgieva, K., Fedina, I., Maslenkova, L. et al., Response of chlorina barley mutants to heat stress under low and high light, Funct. Plant Biol., 2003, 30: 515–524.

    Article  Google Scholar 

  21. Ducruet, J. M., Relation between the heat-induct increase of Fo fluorescence and a shift in the electronic equilibrium at the acceptor side of photosystem 2, Photosynthetica., 1999, 37: 335–338.

    Article  CAS  Google Scholar 

  22. Mishra, R. K., Singhal, G. S., Function of photosynthetic apparatus of intact wheat leaves under high light and heat stress and its relationship with peroxidation of thylakoid lipids, Plant Physiol., 1992, 98: 1–6.

    Article  PubMed  CAS  Google Scholar 

  23. Li, G. F., Mao, H. B., Ruan, X. et al., Association of heat-induced conformational change with activity loss of Rubisco, Biochem. Biophys. Res. Commun., 2002, 290: 1128–1132.

    Article  PubMed  CAS  Google Scholar 

  24. Elrad, D., Niyogi, K. K., Grossman, A. R., A major light harvesting polypeptide of photosystem II function in thermal dissipation, Plant Cell., 2002, 14: 1801–1816.

    Article  PubMed  CAS  Google Scholar 

  25. Andrews, J. R., Fryer, M. J., Baker, N. R., Consequences of LHC II deficiency for photosynthetic regulation in chlorina mutants of barley, Photosynth. Res., 1995, 44: 81–91.

    Article  CAS  Google Scholar 

  26. Breusegem, F. V., Vranová, E., Dat, J. F. et al., The role of active oxygen species in plant signal transduction, Plant Sci., 2001, 161: 405–414.

    Article  Google Scholar 

  27. Foyer, C. H., Lopez-Delgado, H., Dat, J. F., Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signaling, Physiol. Plant., 1997, 100: 241–254.

    Article  CAS  Google Scholar 

  28. Dat, J. F., Lopez-Delgado, H., Foyer, C. H. et al., Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings, Plant Physiol., 1998, 116: 1351–1357.

    Article  PubMed  CAS  Google Scholar 

  29. Kang, H. M., Saltveit, M. E., Antioxidant enzymes and DPPH-radical scavenging activity in chilled and heat-shocked rice (Oryza sativa L.) seedlings radicles, J. Agric. Food Chem., 2002, 50: 513–518.

    Article  PubMed  CAS  Google Scholar 

  30. Pellinen, R. I., Korhonen, M.-S., Tauriainen, A. A. et al., Hydrogen peroxide activates cell death and defense gene expression in birch, Plant Physiol., 2002, 130: 549–560.

    Article  PubMed  CAS  Google Scholar 

  31. Lin, Z. F., Li, S. S., Lin, G. Z. et al., The accumulation of hydrogen peroxide in senescing leaves and chloroplasts in relation to lipid peroxidation, Acta Phytophysiol Sin., 1988, 14: 16–22.

    CAS  Google Scholar 

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Authors and Affiliations

  1. South China Institute of Botany, Chinese Academy of Sciences, Guangdong Key Laboratory of Digital Botanical Garden, 510650, Guangzhou, China

    Zhifang Lin, Changlian Peng, Xinlan Xu & Guizhu Lin

  2. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology & Ecology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, 200032, Shanghai, China

    Jingliu Zhang

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  1. Zhifang Lin
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  2. Changlian Peng
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  3. Xinlan Xu
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  4. Guizhu Lin
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Correspondence to Zhifang Lin.

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Lin, Z., Peng, C., Xu, X. et al. Thermostability of photosynthesis in two new chlorophyllb-less rice mutants. Sci. China Ser. C.-Life Sci. 48, 139–147 (2005). https://doi.org/10.1007/BF02879666

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  • DOI: https://doi.org/10.1007/BF02879666

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