Abstract
Five cultivars ofOenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transformed byAgrobacterium tumefaciens strain which introduces into the plant genome kanamycin resistance gene and the T-DNAipt gene which causes increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, branched shoots with small modified leaves. Roots appeared rarely in later subcultivations of some teratomous clones.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ainley, W.M., McNeil, K.J., Hill, J.W., Linge, W.L., Simpson, R.B., Brenner, M.L., Nagao, R.T., Key, J.L.: Regulatable endogenous production of cytokinins up to ‘toxic’ levels in transgenic plants and plant tissues. - Plant mol. Biol.22: 13–23, 1993.
Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J., Schilperoort, R.A.: A binary plant vector strategy based on separation of vir and T-region of theAgrobacterium tumefaciens Ti plasmid. - Nature303: 179–181, 1983.
Horrobiun, D., Manku, M.: How do polyunsaturated fatty acids lower plasma-cholesterol levels? - Lipids18: 558–562, 1984.
Hudson, B.J.: Evening primrose(Oenothera sp.) oil and seeds. - J. amer. Oil Chem. Soc.61: 540–543, 1984.
Murashige, T.F., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant.15: 473–497, 1962.
Ondřej, M, Hrouda, M., Karavajko, N.N., Matoušek, J., Mikulovič, T.P., Pavingerová, D., Vlasák, J.: Transformation byAgrobacterium vectors and the study of function of plant hormones. - In: Krekule, J., Seidlová, F. (ed.): Signals in Plant Development. Pp. 73–89. SPB Academic Publishing, The Hague 1989.
Ondřej, M., Bavrina, T.V., Dudko, N., Hrouda, M., Krekule, J., Lozhnikova, V., Macháčkóvá, I., Seidlová, F., Vlasák, J.: Transgenic tobacco plants with T-DNA phytohormone synthesizing genes. - Biol. Plant.33: 40–48, 1991.
Stubbe, W., Hermann, R.G.: Selection and maintenance of plastome mutants and interspecific genome/plastome hybrids fromOenothera. - In: Edelman, M., Hallick, R.B., Chua, N.-H. (ed.): Methods in Chloroplast Molecular Biology. Pp. 149–165. Elsevier Biomedical Press, Amsterdam - New York-Oxford 1982.
Van Staden, J., Upford, S., Altaian, A.: Metabolism of [14C] trans-zeatin and [14C] benzyladenine by detached yellow, green and variegated leaves ofSchefflera. - Physiol. Plant.90: 73–78, 1994.
Vlasák, J., Ondřej, M.: Construction and use ofAgrobacterium tumefaciens binary vectors withA. tumefaciens C58 T-DNA genes. - Folia microbiol.37: 227–230, 1992.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pavingerová, D., Gális, I. & Ondřej, M. Tissue culture and transformation ofOenothera biennis . Biol Plant 38, 27–32 (1996). https://doi.org/10.1007/BF02879628
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02879628