Abstract
We have systematically monitored brassinosteroid (BR) responsive genes in a BR-deficient mutantdet2 suspension culture ofArabidopsis by using a cDNA array approach. Among 13000 cDNA clones arrayed on filters, 53 BR responsive clones were identified and designatedBRR1–BRR53. Sequence analysis of 43 clones showed that 19 clones are novel genes, 3 clones are genes involved in the control of cell division, 4 clones are genes related to plant stress responses, 4 clones are transcriptional factor or signal transduction component genes, and 3 clones are genes involved in RNA splicing or structure forming. In addition, we also found that BR regulated the transcription of genes related to many physiological processes, such as photoreaction, ion transportation and some metabolic processes. These findings present molecular evidence that BR plays an essential role in plant growth and development.
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Grove, M. D., Spencer, G. F., Rohwedder, W. K. et al., Brassinolide, a plant growth-promoting steroid isolated from Brassica napus pollen, Nature, 1979, 281: 216–217.
Mandava, N. B., Plant growth-promoting brassinosteroids, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1988, 39: 23–52.
Clouse, S. D., Sasse, J. M., Brassinosteroids: essential regulators of plant growth and development, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1998, 49: 427–451.
Altmann, T., Recent advances in brassinosteroid molecular genetics, Curr. Opin. Plant Biol., 1998, 1: 378–383.
Aharoni, A., Keizer, L. C. P., Bouwmeester, H. J. et al., Identification of the SAAT gene involved in strawberry flavor biogenesis by use of DNA microarray, Plant Cell, 2000, 12: 647–661.
Reymond, P., Weber, H., Damond, M. et al., Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis, Plant Cell, 2000, 12: 707–719.
Hu, Y., Han, C., Mou, Z. et al., Monitoring gene expression by cDNA array, Chin. Sci. Bull., 1999, 44: 441–444.
Fujioka, S., Li, J., Choi, Y. H. et al., The Arabidopsisdeetiolated2 mutant is blocked early in brassinosteroid biosynthesis, Plant Cell, 1997, 9: 1951–1962.
Wadsworth, G. J., Redinbaugh, M. G., Scandalios, J. G., A procedure for small-scale isolation of plant RNA suitable for RNA blot analysis, Anal. Biochem., 1988, 172: 279–283.
Church, G. M., Gilbert, W., Genomic sequencing, Proc. Natl. Acad. Sci. USA, 1984, 81: 1991–1995.
Huntley, R. P., Murray, J. A. H., The plant cell cycle, Curr. Opin. Plant Biol., 1999, 2: 440–446.
Riou-Khamlichi, C., Huntley, R., Jacqmard, A. et al., Cytokinin activation of Arabidopsis cell division through a D-type cyclin, Science, 1999, 283: 1541–1544.
Hu, Y., Bao, F., Li, J., Promotive effect of brassinosteroids on cell division involves a distinct CycD3-induction pathway, Plant J., 2000, 24: 693–701.
Hirayama, T., Shinozaki, K., A cdc5+ homolog of a higher plant, Arabidopsis thaliana, Proc. Natl. Acad. Sci. USA, 1996, 93: 13371–13376.
Javerzat, J. P., Cranston, G., Allshire, R. C., Fission yeast genes which disrupt mitotic chromosome segregation when overexpressed, Nucl. Acids Res., 1996, 24: 4676–4683.
Dhaubhadel, S., Chaudhary, S., Dobinson, K. F. et al., Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance ofBrassica napus and tomato seedlings, Plant Mol. Biol., 1999, 40: 333–342.
Beato, M., Herrlich, P., Schytz, G., Steroid hormone receptors: many actors in search of a plot, Cell, 1995, 83: 153–156.
Chou, I. T., Gasser, C. S., Characterization of the cyclophilin gene familyof Arabidopsis thaliana and physiologenetic analysis of known cyclophilin proteins, Plant Mol. Biol., 1997, 35: 873–892.
Sykes, K., Gething, M. J., Sambrook, J., Proline isomerases function during heat shock, Proc. Natl. Acad. Sci. USA, 1993, 90: 5853–5857.
Marivet, J., Frendo, P., Burkard, G., DNA sequence analysis of a cyclophilin gene from maize: developmental expression and regulation by salicylic acid, Mol. Gen. Genet., 1995, 247: 222–228.
Kagaya, Y., Ohmiya, K., Hattori, T., RAV1, a novel DNA-binding protein, binds to bipartite recognition sequence through two distinct DNA-binding domains uniquely found in higher plant, Nucleic Acids Res., 1999, 27: 470–478.
Neff, M. M., Nguyen, S. M., Malancharuvil, E. J. et al.,BAS1: a gene regulating brassinosteroid levels and light responsiveness in Arabidopsis, Proc. Natl. Acad. Sci. USA, 1999, 96: 15316–15323.
Chory, J., Nagpal, P., Peto, C. A., Phenotypic and genetic analysis ofdet2, a new mutant that affects light-regulated seedling development in Arabidopsis, Plant Cell, 1991, 3: 445–459.
Papenbrock, J., Grafe, S., Kruse, E. et al., Mg2+-chelatase of tobacco: identification of aChlD cDNA sequence encoding a third subunit, analysis of the interaction of the three subunits with the yeast two-hybrid system, and reconstitution of the enzyme activity by co-expression of recombinant ChlD, ChlH and ChlI, Plant J., 1997, 12: 981–990.
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Hu, Y., Wang, Z., Wang, Y. et al. Identification of brassinosteroid responsive genes inArabidopsis by cDNA array. Sci. China Ser. C.-Life Sci. 44, 637–643 (2001). https://doi.org/10.1007/BF02879358
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DOI: https://doi.org/10.1007/BF02879358