Skip to main content
SpringerLink
Log in

Effect of phosphatidylglycerol on conformation and micro-environment of tyrosyl residue in photosystem II

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

The structural aspects in the interaction of phosphatidylglycerol (PG) with photosystem II (PSII), mainly the effect of PG on conformation and microenvironment of tyrosine residues of PSII proteins were studied by Fourier transform infrared (FTIR) spectroscopy. It was found that the binding of PG to PSII particle induces changes in the conformation and micropolarity of phenol ring in the tyrosine residues. In other words, the PG effect on the PSII results in blue shift of the stretch vibrational band in the phenol ring from 1620 to 1500 cm−1 with the enhancement of the absorbance intensity. Additionally, a new spectrum of hydrogen bond was also observed. The results imply that the hydrogen-bond formation between the OH group of phenol and one of PG might cause changes in the structures of tyrosine residues in PSII proteins.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Webb, M. S., Green, B. R., Biochemical and biophysical properties of thylakoid acyl lipids, Biochim. Biophys. Acta, 1991, 1060: 133.

    Article  CAS  Google Scholar 

  2. Joyard, J., Maréchal, E., Miège, C. et al., Structure, distribution and biosynthesis of glycerolipids from higher plant chloroplast, in Lipids in Photosynthesis: Structure, Function and Genetics (eds. Siegenthaler, P. A., Murata, M.), The Netherlands: Kluwer Academic Publishers, 1998, 21–52.

    Google Scholar 

  3. Nußberger, S., Dörr, K., Wang, D. N. et al., Lipid-protein interaction in crystals of plant light-harvesting complex, J. Mol. Biol., 1993, 224: 347.

    Article  Google Scholar 

  4. Siegenthaler, P. A., Rawyler, A., Smutny, J., The phospholipid population which sustains the uncoupled non-cyclic electron flow activity is localised in the inner monolay of the thylakoid membrane, Biochim. Biophys. Acta, 1989, 975: 104.

    Article  CAS  Google Scholar 

  5. Murata, M., Higashi, S., Fujimura, Y., Glycerolipids in various preparations of photosystem II from spinach chloroplasts, Biochim. Biophys. Acta, 1990, 1019: 261.

    Article  CAS  Google Scholar 

  6. Kruse, O., Schmid, G. H., The role of phosphatidylglycerol as a functional effector and membrane anchor of the D1-core peptide from photosystem II particles of the cyanobacteriumOscillatoria chalybea, Z Naturforsch, 1995, 50c: 380.

    Google Scholar 

  7. Yang Zhenle et al., Effect of phosphatidylglycerol on oxygen evolution in photosystem II, Acta Botany Sinica, 2000, 42(3): 249.

    Google Scholar 

  8. Berthold, D. A., Babcick, G. T., Yocum, C. F., A highly resolved, oxygen-evolving photosystem II preparation from spinach thylakoid membranes, EPR and electron-transport properties, FEBS Lett., 1981, 134: 231.

    Article  CAS  Google Scholar 

  9. Arnon, D. I., Copper enzymes in isolated chloroplasts: polyphenyloxidase inBeta vulgaris, Plant Physiol., 1949, 14: 1.

    Google Scholar 

  10. Katz, J. J., Diugherty, R. C., Boucher, L. J., Infrared and nuclear magnetic resonance of chlorophyll, in The Chlorophylls (eds. Vemon, L. P., Seely, G. R.), New York: Academic Press, 1966, 185–251.

    Google Scholar 

  11. Jackson, M., Mantsch, H.H., Biomembrane structure from FTIR spectroscopy, Spectrochimica Acta Rev., 1993, 15: 53.

    CAS  Google Scholar 

  12. He, W. Z., Newell, W. R., Haris, P. I. et al., Protein secondary structure of the isolated photosystem II reaction center and conformational changes studied by Fourier transformation infrared spectroscopy, Biochemistry, 1992, 31: 9848.

    Article  Google Scholar 

  13. Byler, D. M., Susi, H., Examination of the secondary structure of protein by deconvolved FTIR spectra, Biopolymers, 1986, 25: 469.

    Article  PubMed  CAS  Google Scholar 

  14. MacDonald, G. M., Barry, B. A., Difference FTIR study of a novel biochemical preparation of photosystem II, Biochemistry, 1992, 31: 9848.

    Article  PubMed  CAS  Google Scholar 

  15. Yao Xinsheng, Chen Yingjie, Xu Suixu et al., Spectral Analysis of Organic Compounds (in Chinese), Beijing: People’s Sanitation Press, 1981.

  16. Amesz, J., Hoff, A. J., eds., Biophysical Techniques in Photosynthesis, Dordrecht: Kluwer Academic Publishers, 1996.

    Google Scholar 

  17. Susi, H., Byler, D. M., Resolution-enhanced Fourier transform infrared spectroscopy of enzymes, Methods in Enzymology, 1986, 130: 290.

    Article  PubMed  CAS  Google Scholar 

  18. Chirgadze, Y. N., Fedorov, O. V., Trushina, N. P., Estimation of amino acid residue side chain absorption in the infrared spectra of protein solutions in heavy water, Biopolymers, 1975, 14: 679.

    Article  PubMed  CAS  Google Scholar 

  19. Wilson, E. B., Decius, J. C., Cross, P. C., The Theory of Infrared and Ramam Vibrational Spectra, New York: McGraw-Hill, 1955.

    Google Scholar 

  20. Venyaminov, S. Y., Kalnin, N. N., Quantitative IR spectrophotometry of peptide compounds in water (H2O) solution (I) Spectral parameters of amino acid residue absorptions, Biopolymers, 1990, 30: 1243.

    Article  PubMed  CAS  Google Scholar 

  21. Colthup, N. B., Daly, L. H., Wiberley, S. E., Introduction to Infrared and Raman Spectroscopy, 3rd ed., Boston: Academic Press, 1990.

    Google Scholar 

  22. Paiter, P. C., Coleman, M. M., Koenig, J. L., The Theory of Vibrational Spectroscopy and Its Applications to Polymeric Materials, New York: Wiley-Interscience, 1962.

    Google Scholar 

  23. Fragata, M., Nenonene, E. K., Maire, V. et al., Structure of the phosphatidylglycerol-photosystem II complex studied by FT-IR spectroscopy, Mg(II) effect on the polar head group of phosphatidylglycerol, J. Mol. Struct., 1997, 405: 151.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences, 100093, Beijing, China

    Zhenle Yang, Liangbi Li, Yinong Xu & Tingyun Kuang

Authors
  1. Zhenle Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Liangbi Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yinong Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tingyun Kuang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhenle Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, Z., Li, L., Xu, Y. et al. Effect of phosphatidylglycerol on conformation and micro-environment of tyrosyl residue in photosystem II. Sci. China Ser. C.-Life Sci. 43, 330–336 (2000). https://doi.org/10.1007/BF02879293

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02879293

Keywords

Search

Navigation