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In vitro flowering ofDendrobium candidum

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Abstract

Dendrobium candidum, a wild orchid species from China, normally requires three to four years of cultivation before it can produce flowers. The effects of plant hormones and polyamines on flower initiation of this species in tissue culture were investigated. The addition of spermidine, or BA, or the combination of NAA and BA to the culture medium can induce protocorms or shoots to flower within three to six months with a frequency of 31.6%-45.8%. The flowering frequency can be further increased to 82.8 % on the average by pre-treatment of protocorms in an ABA-containing medium followed by transfer onto MS medium with BA. The induction of precocious flowering depends on the developmental stage of the experimental materials (protocorms, shoots and plantlets) used, and usually occurs only when mt formation is inhibited.

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Abbreviations

ABA:

Abscisic acid

BA:

6-benzylaminopurine

NAA:

naphthlene acetic acid

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Authors and Affiliations

  1. Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, 200032, Shanghai, China

    Guangyuan Wang & Zhihong Xu

  2. Institute of Molecular and Cell Biology, National University of Singapore, 0511, Singapore, Republic of Singapore

    Guangyuan Wang, Zhihong Xu & Tet-Fatt Chia

  3. Laboratory of Plant Molecular Biology, The Rockefeller University, 10021-6399, N.Y., USA

    Nam-Hai Chua

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  1. Guangyuan Wang
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  2. Zhihong Xu
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  3. Tet-Fatt Chia
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  4. Nam-Hai Chua
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Wang, G., Xu, Z., Chia, TF. et al. In vitro flowering ofDendrobium candidum . Sci. China Ser. C.-Life Sci. 40, 35–42 (1997). https://doi.org/10.1007/BF02879105

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  • DOI: https://doi.org/10.1007/BF02879105

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