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Double-gating mechanism and diversity of an adenosine triphosphate (ATP)-sensitive K+ channel in neurons acutely dissociated from rat neocortex

Science in China Series C: Life Sciences volume 40, pages 10–17 (1997)Cite this article

Abstract

Classically, ion channels are classified into 2 groups: chemical-sensitive (ligand-gated) and voltage sensitive channels. Single ATP-sensitive K+ (K-ATP) channel currents were recorded in acutely dissociated rat neocortical neurons using patch clamp technique. A type of K-ATP channel has been found to be gated not only by intracellular ATP, but also by membrane potential (Vm), and proved to be a novel mechanism underlying the gating of ion channels, namely bi-gating mechanism. The results also show that the K-ATP channels possess heterogeneity and diversity. These types of K-ATP channels have been identified in 40.12% of all patches, which are different in activation-threshold and voltage-sensitivity. The present experiment studied the type-3 K-ATP channel with a unitary conductance of about 80 pS in detail (n = 15). Taking account of all the available data, a variety of K-ATP channels are suggested to exist in body, and one type of them is bi-gated by both chemical substances and membrane potentials. This property of the K-ATP channels may be related to their pathophysiological function.

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Author notes

  1. Wenjun Yang

    Present address: Department of Neurology, Nanfang Hospital, 510515, Guangzhou, China

Affiliations

  1. Department of Physiology, First Military Medical University, 510515, Guangzhou, China

    Zhenqing Tong, Xiangdong Tang & Wenjun Yang

Authors
  1. Zhenqing Tong
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  2. Xiangdong Tang
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  3. Wenjun Yang
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Project supported by the National Natural Science Foundation of China and Natural Science Foundation of Guangdong Province

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Tong, Z., Tang, X. & Yang, W. Double-gating mechanism and diversity of an adenosine triphosphate (ATP)-sensitive K+ channel in neurons acutely dissociated from rat neocortex. Sci. China Ser. C.-Life Sci. 40, 10–17 (1997). https://doi.org/10.1007/BF02879102

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  • DOI: https://doi.org/10.1007/BF02879102

Keywords

  • ATP-sensitive K+ channel
  • gating kinetics
  • patch clamp
  • dissociated neuron
  • rat