Abstract
Two barley chloroplast nuclease fractions were separated by the affinity chromatography and gel electrophoresis. Both were about 2 times more active to RNA than to native DNA and about half as active to denaturated DNA as to native DNA. Both fractions were as active to UV-irradiated (270 J m-2) native DNA as to intact DNA but their action was inhibited by apurinic sites. The enzyme activities were inhibited by high concentrations of EDTA, NaCl, Mn2+, Ca2+, Zn2+ ions and by N-ethylmaleimide. They do not require Mg2+ ions but are stimulated or at higher concentration inhibited by their presence. Both RNase and DNase were active over a wide pH range (5.5–9), the optimum for DNase action in the presence of Mg2+ being 6.5, for RNA decomposing activity at pH 8.0. As no mononucleotides were detected in acid soluble form, it seems likely that DNase acts in the endonucleolytic way.
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Švachulová, J., Velemínský, J. & Šatava, J. Nucleases in chloroplast of barley. Biol Plant 22, 143–151 (1980). https://doi.org/10.1007/BF02878255
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DOI: https://doi.org/10.1007/BF02878255