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Proton extrusion by Leaf Discs ofVicia faba L.: Influence of inhibitors and phytohormones on H+ extrusion†

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Biologia Plantarum

Abstract

The present investigations were designed to elucidate the linkage of medium acidification to metabolism and to study the influence of phytohormones (IAA, ABA) and fusieoccin (FC) on H+ extrusion from peeled leaf tissue of Vicia faba. H+ release, which was sensitive to the metabolic inhibitors sodium azide, vanadate, DCCD, and cycloheximide, was promoted by 5 to 50 μM IAA and 10 μ FC, but transiently diminished by 10 μM ABA. FC was a much more powerful inducer of H+ extrusion than IAA. Other weak acids such as acetate and citrate had no effect on H+ extruding activity of Vicia leaf tissue indicating that the IAA effect is specific. These results support the view that IAA and ABA control of cell growth can be attributed entirely to a modulation of H+ pump activity.

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Authors and Affiliations

  1. Sektion Biowissenschaften, WB Pflanzenphysiologie, Martin-Luther-Universität Halle-Wittenberg, Am Kirehtor 1, 4020, Halle

    U. Petzold & P. Jacob

  2. Sektion Biologic, WB Biophysik, Friedrich-Schiller-Universität Jena, Philosophenweg 14a, 6900, GDR, Jena

    I. Dahse

Authors
  1. U. Petzold
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  2. I. Dahse
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  3. P. Jacob
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Petzold, U., Dahse, I. & Jacob, P. Proton extrusion by Leaf Discs ofVicia faba L.: Influence of inhibitors and phytohormones on H+ extrusion†. Biol Plant 30, 252–259 (1988). https://doi.org/10.1007/BF02878203

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  • DOI: https://doi.org/10.1007/BF02878203

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