Abstract
Supplement of liquid culture medium with 5 % (v/v) of dimethylsulfoxide (DMSO) for two subcultivation intervals increased permeability of cell suspension culture of Nicotiana tabactim L. This effect resulted in reduction of fresh mass yield, increase of relative protein content and release of protein into medium during the first subcultivation. Permeabilized cells were further cultivated either in DMSO-free or DMSO-containing medium. Recovery of cells occurred in the former medium characterized by an increase in fresh mass and changes in content and excretion of proteins similar to that found during the first subcultivation in presence of DMSO. Cells cultured for the two subcultivation intervals in DMSO-containing medium undergo a physiological stress and gradually die out. It is evident that DMSO cannot be used as permeabilization agont in long term experiments.
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Meravý, L., Cvikrová, M. & Hrtjbcová, M. The effect of DMSO on excretion of proteins and phenolic substances byNicotiana tabacum cells into culture medium. Biol Plant 30, 241–246 (1988). https://doi.org/10.1007/BF02878201
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DOI: https://doi.org/10.1007/BF02878201