Abstract
The content and changes in the activity of phosphogluconate dehydrogenase were followed in leaf tissues of tobacco plants infected with the potato virus Y (PVY) in the acute phase of infection. The activity of the enzyme was higher in virus-infected tissues during the entire experimental period compared with the values found in healthy control plants in both crude homogenate and partially purified enzyme preparation. The courses of the activity curves of both the crude extract and partially purified enzyme preparations were consistent and correlated with the reproduction curve of the virus. These results suggest that increased activity of the enzyme was a result of its coarse regulation and indicate its immediate involvement in“de novo” biosynthesis of the virus via the oxidative pentose phosphate pathway.
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Šindelář, L., Šindelářová, M. Changes in the activity of phosphogluconate dehydrogenase and its regulation in tobacco infected with PVY. Biol Plant 29, 150–154 (1987). https://doi.org/10.1007/BF02878170
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DOI: https://doi.org/10.1007/BF02878170