Abstract
A histochemical method for the determination of IAA-oxidase has been used in sections of various aerial parts of winter wheat plants. High IAA-oxidase activity was localized in the cell walls of sclerenchyma near the periphery of the stem, in the vascular bundle sheath of sclerenchyma and in xylem, both in the stem and in the leaf. The cell wall—bound IAA-oxidase activity therefore appeared in lignifying tissues. The staining was very weak or absent in the cell walls of parenchyma tissues and phloem. The positive reaction of the cytosol at the bulbous ends of guard cells and in the leaf primordia is presumed to be due to cytosolic IAA-oxidase. These results are discussed in relation to peroxidase localization and to our previousin vitro studies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beneš, K., SeidlovÁ, F.: A suitable method for localization of IAA oxidase. — Biol. Plant.20: 64–66, 1978.
Catesson, A. M.: Localization of phloem oxidases. — Ber. deut. bot. Ges.93: 141–152, 1980.
Czaninski, Y.: Localisation ultrastructurale d’activités peroxydasiques dans les parois du xylème du Blé pendant leur différentiation. — Compt. rend. Acad. Sci. Paris286: 957–959, 1978.
Esau, K.: Anatomy of Seed Plants. — John Wiley and Sons, New York 1977.
Gaspar, T., Penel, C., Thorpe, T., Greppin, H.: Peroxidases 1970—1980. — Univ. de Genève, Centre de Botanique, Genéve 1982.
Goldberg, R., Catesson, A. M., Czaninski, Y.: Some properties of syringaldazine oxidase, a peroxidase specifically involved in the lignification processes. — Z. Pflanzenphysiol.110: 267–279, 1983.
Hall, J. L., Sexton, R.: Cytochemical localization of peroxidase activity in root cells. — Planta108: 103–120, 1972.
Harche, M.: Localisation struoturale et ultrastructurale d’activités peroxydasiques dans les parois du mesophylle et des fibres en cours de lignifieation chez l’alfa (Stipa tenacissima).— Can. J. Bot.62: 2644–2649, 1984.
Harkin, J. M., Obst, Y. R.: Lignification in trees: indication of exclusive peroxidase participation. — Science180: 296–298, 1973.
MachÁČKOVÁ, I., GanČeva, K., Zmrhal, Z.: The role of peroxidase in the metabolism of indole-3-acetic acid and phenols in wheat. — Phytochemistry14: 1251–1254, 1975.
MÄder, M., Amberg-Fischer, V.: Role of peroxidase in lignification of tobacco cells. I. Oxidation of nicotinamide adenine dinucleotide and formation of hydrogen peroxide by cell wall peroxidases. — Plant Physiol.70: 1128–1131, 1982.
Meudt, W. J., Gaines, T. P.: Studies on the oxidation of indole-3-acetic acid by peroxidase enzymes. I. Colorimetric determination of indole-3-acetic acid oxidation products. — Plant. Physiol.42: 1395–1399, 1967.
SÁgi, F.: Cell wall-bound indoleacetic acid oxidases in hypocotyls of the white lupin (Lupinus albus L.). — In: Abstracts of International Conference of Natural Plant Growth Substances. Pp. 85–86. Liblice 1972.
Stonier, T., Stasinos, S., Reddy, K. B. S. M.: The masking of peroxidase-catalyzed oxidation of IAA inVigna. — Phytochemistry18: 25–28, 1979.
Waldrum, J. D., Davies, E.: Subcellular localization of IAA oxidase in peas. — Plant Physiol.68: 1303–1307, 1981.
Zmrhal, Z., MaohÁČkovÁ, I.: Isolation and characterization of wheat peroxidase isoenzyme B 1. — Phytochemistry17: 1517–1520, 1978.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zmrhal, Z., Seidlová, F. & Macháčková, I. Histochemical IAA-oxidase localization in the shoot of wheat. Biol Plant 29, 94–97 (1987). https://doi.org/10.1007/BF02878157
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02878157