Skip to main content
Log in

Utilization of exogenous sugars by excised maize embryos in culture

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Sucrose was markedly superior to fructose and glucose in promoting growth of plantlets from immature maize embryos. The elongation of roots is shown to be more sucrose dependent than that of shoots. On the other hand, the exogenous sucrose was less effective than fructose as substrate for carbohydrate catabolism and for the synthesis of alcohol-insoluble compounds at the beginning of embryo cultivation. The absorbed fructose was found to be rapidly converted to sucrose and the level of endogenous sucrose derived from sugar supplied to the medium was higher in fructosethan in sucrose-fed embryos. The preferential utilization of fructose over sucrose, however, declined with the progress of germination which may be related to the decrease in proportion of scutellum in total mass and physiological activity of the embryo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Reference

  • Barcelrlona-Vero, L., Mino, L., Oddo, N., Reali-Tempesta, P., Tonolo, A.: Sterile submerged culture of some Graminaceous plantlets. — Experientia24: 1288–1289, 1968.

    Article  Google Scholar 

  • Burghardtová, K., Tupý, J.: Invertase of scutellum and embryo axis in maize embryos. — Biológia (Bratislava)34: 335–338, 1979.

    Google Scholar 

  • Edelman, J., Shibko, S. I., Keys, A. J.: The role of the scutellum of cereal seedlings in the synthesis and transport of sucrose. — J. exp. Bot.10: 178–189, 1959.

    Article  CAS  Google Scholar 

  • Humphreys, T. E., Gardard, L. A.: Glucose uptake by the corn scutellum. — Phytochemistry3: 647–656, 1964.

    Article  CAS  Google Scholar 

  • Humphreys, T. E., Gareard, L. A.: Leakage and storage of sucrose during sucrose synthesis in the corn scutellum. — Phytochemistry5: 653–663, 1966.

    Article  CAS  Google Scholar 

  • Murray, J.: Liquid scintillation counting of14CC2 in a toluene/triton X-100 system. — Int. J. appl. Rad. Isotop.22: 200–212, 1971.

    Google Scholar 

  • Nygaard, P.: Utilization of exogenous carbohydrates for tube growth and starch synthesis in pine pollen suspension cultures. — Physiol. Plant.39: 206–210, 1977.

    Article  CAS  Google Scholar 

  • Purvis, O. N.: Studies in the vernalisation of cereals. VIII. The role of oarbohydrate and nitrogen supply in the vernalisation of excised embryos of “Petkus” winter rye. — Ann, Bot. N.S.8: 285–314, 1944.

    CAS  Google Scholar 

  • Raghavan, V.: Experimental Embryogenesis in Vascular Plants. — Academic Press, London— —New York—San Francisco 1976.

    Google Scholar 

  • Street, H. E.: Growth in organized and unorganized system. — In:Steward, F. C. (ed.): Plant Physiology — A Treatise. Vol. 5B. Pp. 3–225. Academic Press, New York—London 1969.

    Google Scholar 

  • Thomas, D. R., Craigie, J. S., Street, H. E.: Carbohydrate nutrition of the excised tomato root. — Int. Soc. Plant Morphologists, Univ. of Delhi, Delhi 1963.

    Google Scholar 

  • Tupý, J.: Radiorespirometrio study of the utilization of exogenous sucrose, glucose and fructose by germinating apple pollen. — Biol. Plant.4: 69–84, 1962.

    Article  Google Scholar 

  • Vítek, V.: Sugar-like, diphenylamine-positive metabolites in pea seedlings. — Biochim. biophys. Acta93: 429–432, 1964.

    PubMed  Google Scholar 

  • Whitesell, J. H., Humphreys, T. E.: Sugar uptake in the maize soutellum. — Phytoohemistry11: 2139–2147, 1972.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Burghardtová, K., Tupý, J. Utilization of exogenous sugars by excised maize embryos in culture. Biol Plant 22, 57–64 (1980). https://doi.org/10.1007/BF02878128

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02878128

Keywords

Navigation