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Comparison of seed proteins of some representatives of the genusPisum from the point of view of their relationship comparison by disc electrophoresis

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Biologia Plantarum

Abstract

Ten taxa of the genusPisum were examined by disc electrophoresis in gels according to Davis and to Reisfeldet al. For evaluation of band patterns the Jaccard Index was applied.

The results in both types of gels show thatPisum abyssinicum and especiallyP. fulvum have biochemically a relatively isolated position.Pisum elatius and its subspeciescaspicum andpalestinicum form a subgroup withP. cinereum;P. sativum var.zeylanicum and cv. Jupiter form another subgroup withP. syriacum.

Our results are in good agreement with the results of Przybylskaet al. (with the exception ofP. cinereum) and also with immunoelectrophoretic analyses performed by Turkováet al. (1980), with the same exception.

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Abbreviations

PAGE:

Polyaorylamide gel electrophoresis

References

  • Boulter, D., Thurman, D. A.: Acrylamide gel electrophoresis of proteins in plant systematics. — Systematics Assoc. Spec. Vol.2: 39–48, 1968.

    CAS  Google Scholar 

  • Boulter, D., Thurman, D. A., Derbyshire, E.: A disc electrophoretic study of globulin proteins of legume seeds with reference to their systematics. — New Phytol.66: 27–36, 1967.

    Article  CAS  Google Scholar 

  • Boulter, D., Thurman, D. A., Turner, B. L.: The use of disc electrophoresis of plant proteins in systematics. — Taxon15: 135–143, 1966.

    Article  Google Scholar 

  • Chumikina, L. V., Gumilevskaya, N. A., Kretovich, V. L.: Investigation of ribosomal proteins and ribosomal subunits of pea seeds by electrophoresis in polyacrylamide gels. — Biokhimia40: 267–275, 1975.

    CAS  Google Scholar 

  • Davis, B. J.: Disc electrophoresis. — Ann. N. Y. Acad. Sci.121: 404–427, 1964.

    Article  PubMed  CAS  Google Scholar 

  • Fox, D. J., Thurman, D. A., Boulter, D.: Studies on the proteins of seeds of theLeguminosae. — Phytochemistry3: 417–419, 1964.

    Article  CAS  Google Scholar 

  • Hall, T. C., McCown, B. H., Desborough, S., McLeester, R. C., Beck, C. E.: A comparative investigation of isozyme fractions separated from plant tissues. — Phytochemistry8: 385 to 391, 1969.

    Article  Google Scholar 

  • Hart, G. E., Bhatia, C. R.: Acrylamide gel electrophoresis of soluble leaf proteins and enzymes fromNicotiana species. — Can. J. Genet. Cytol.9: 367–374, 1967.

    CAS  Google Scholar 

  • Hull, P.: Differences in esterase distribution detected by electrophoresis as evidence of continuing interspecific hybridization in the genusSenecio. — Ann. Bot.38: 697–700, 1974.

    Google Scholar 

  • Jaccard, P.: Die statistisch-floristische Methode als Grundlage der Pflanzensoziologie. — In: Abderhalden, E. (ed.): Handbuch der biologischen Arbeitsmethoden. Berlin — Wien 1928.

  • Maršálek, L.: Study in heredity of the spectrum of peroxidase isoenzymes with genotypes of the F1 and F2 generations following the diallele crossing of self-pollinated lines of maize. — Acta Univ. Agr. Fac. Agron. (Praha)22: 433–441, 1974.

    Google Scholar 

  • Mitra, R., Jagannath, D. R., Bhatia, R.: Disc electrophoresis of analogous enzymes inHordeum. — Phytochemistry9: 1843–1850, 1970.

    Article  CAS  Google Scholar 

  • Przybylska, J., Blixt, S., Hurich, J., Zimniak-Przybylska, Z.: Comparative study of seed proteins in the genusPisum. — Genet. pol.18: 27–38, 1977.

    CAS  Google Scholar 

  • Reisfeld, R. A., Lewis, V. J., Williams, D. E.: Disc electrophoresis of basic proteins and peptides on polyacrylamide gels. — Nature195: 281–283, 1962.

    Article  PubMed  CAS  Google Scholar 

  • Safonov, V. I., Veïdenberg, A. E.: [Non-identity of proteins and enzyme systems as a factor of the physiological incompatibility in apple at crossing and grafting.] In Russ. — Fiziol. Rast.19: 810–818, 1969.

    Google Scholar 

  • Šašek, A., Černý, J.: Verification of wheat mutants (Triticum aestivum L.) by means of gliadin spectra. — Rost. Výroba (Praha)24: 517–525, 1978a.

    Google Scholar 

  • Šašek, A., Černý, J., Folk, A.: Electrophoretic analysis of seminal proteins in oilseed rape (Brassica napus L.). — Rost. Výroba (Praha):24: 409–417, 1978b.

    Google Scholar 

  • Turková, V., Klozová, E., Hadačová, V.: The comparison of seed proteins of several representatives of the genusPisum with respect to their relationships. An immunological comparison. — Biol. Plant.22: 17–24, 1980.

    Google Scholar 

  • Vaughan, J. G., Denford, K. G.: An acrylamide gel electrophoretic study of the seed proteins ofBrassica andSinapis species, with special reference to their taxonomic value. — J. exp. Bot.19: 724–732, 1968.

    Article  CAS  Google Scholar 

  • Vaughan, J. G., Waite, A., Boulter, D., Waiters, S.: Taxonomic investigation of severalBrassica species using serology and the separation of proteins by electrophoresis on gels. — Nature208: 704–705, 1965.

    Article  Google Scholar 

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Hadačová, V., Turková, V., Hadač, E. et al. Comparison of seed proteins of some representatives of the genusPisum from the point of view of their relationship comparison by disc electrophoresis. Biol Plant 22, 7–16 (1980). https://doi.org/10.1007/BF02878122

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  • DOI: https://doi.org/10.1007/BF02878122

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