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Plant proteases as interfering factors in the electron microscopic detection of alfalfa mosaic and bean yellow mosaic viruses inSolanum laciniatum Ait

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Biologia Plantarum

Abstract

Plant proteases rapidly destroy virus architecture in sap ofSolanum laciniatum Ait., with yellow mosaic symptoms but even under these conditions the partially digested amorphous nucleoproteins retain their immunospecificity and infectivity for a limited time. Homogenization with phenylmethylsulfonylfluoride inhibits efficiently host serine proteases and fully stabilizes the virus particles in plant sap and in partial purificates. During the electronmicroscopic examination of stabilized infectious saps in 12 out of 14 isolates of yellow mosaic ofS. laciniatum besides alfalfa mosaic virus a poty-virus was disclosed. This was identified serologically and by transmission tests as a weakly virulent type of bean yellow mosaic virus.

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Authors and Affiliations

  1. Institute of Experimental Botany, Czechoslovak Academy of Sciences, Na Karlovce 1, 160 00, Praha 6, Czechoslovakia

    M. Cech

  2. Research Institute of Pharmacology and Biochemistry, Praha

    S. Neubaur

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  1. M. Cech
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  2. S. Neubaur
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Cech, M., Neubaur, S. Plant proteases as interfering factors in the electron microscopic detection of alfalfa mosaic and bean yellow mosaic viruses inSolanum laciniatum Ait. Biol Plant 23, 384–388 (1981). https://doi.org/10.1007/BF02877420

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  • DOI: https://doi.org/10.1007/BF02877420

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