Abstract
When investigating migration and chemotaxis of leukocytes under agarose polyvinylpyrrolidone (PVP; 11 kg/mol) and polyethyleneglycol (PEG; 6 kg/mol) were used instead of serum. The highest migration was detected with 0.5–1 mm of PVP. Phagocytosis was not influenced either by PVP or PEG within a concentration range of 0.01 – 1 mM.
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References
Boyden S.: Chemotactic effect of mixture of antibody and antigen on polymorphonuclear leukocytes.J.Exp.Med. 115, 453 (1962).
Cutler J.E.: A simplein vitro method for studies on chemotaxis.Proc.Soc.Exp.Biol.Med. 147, 471 (1974).
Czarnetzki B.M., Schulz W.: Role of purified serum components in polymorphonuclear leukocyte chemotaxis.Int.Arch.Allergy Appl.Immunol. 61, 424 (1980).
Leonard E.J., Skeel A.: A serum protein that stimulated macrophage movement, chemotaxis and spreading.Exp.Cell. Res. 102, 434 (1976).
Nelson R.D., Quie P.G., Simmons R.L.: Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes.J. Immunol. 115, 1650 (1975).
Palisa V., Krigar V., Mainz J.: A method of phagocytic activityin vitro. (In Czech).Immunological bulletin 9, 42(1978).
Repo H.: Leukocyte migration agarose test for the assessment of human neutrophil chemotaxis. I. Effects of environmental factors on neutrophil migration under agarose.Scand. J. Immunol. 6, 203 (1977).
Till G., Ward P.A.: Two distinct chemotactic factors inactivators in human serum.J. Immunol. 114, 843 (1976).
Wilkinson P.C.: A requirement for albumin as carries for low molecular weight leukocyte chemotactic factors.Exp.Cell.Res. 103, 415 (1976).
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Sýkora, J., Stajner, A., Palisa, V. et al. Migration of leukocytes under agarose in the presence of some polymers. Folia Microbiol 27, 214–216 (1982). https://doi.org/10.1007/BF02877403
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DOI: https://doi.org/10.1007/BF02877403