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Role of phosphatases in the biosynthesis of chlortetracycline inStreptomyces aureofaciens

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Abstract

ATP diphosphohydrolase activity and inorganic pyrophosphatase reached two maxima during cultivation of the low- and high-producing variant ofStreptomyces aureofaciens under conditions of phosphate limitation,i.e. after 30 and 70 h of cultivation. Increased levels of inorganic phosphate in a medium inhibitory to biosynthesis of chlortetracycline markedly decreased the levels of both enzymes. The ATP diphosphohydrolase activity was detected both in the supernatant and membrane fractions of the cell-free preparation of the mycelium.

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Authors and Affiliations

  1. Department of Biogenesis ot Natural Products, Institute of Microbiology, Czechoslovak Academy of Sciences, 142 20, Prague 4

    V. Jechová, E. Čurdová & Z. Hošťálek

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  1. V. Jechová
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  2. E. Čurdová
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  3. Z. Hošťálek
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Jechová, V., Čurdová, E. & Hošťálek, Z. Role of phosphatases in the biosynthesis of chlortetracycline inStreptomyces aureofaciens . Folia Microbiol 27, 153–158 (1982). https://doi.org/10.1007/BF02877393

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  • DOI: https://doi.org/10.1007/BF02877393

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