Abstract
Simultaneous reduction in alkaloid yield and level of phosphatases by high concentrations of phosphate was observed inClaviceps sp. SD-58. Tryptophan-induced culture showed an increase in alkaloid yield and the level of phosphatases. Phosphate caused repression of both acid phosphatase (isoenzyme I) and alkaline phosphatase (isoenzymes III and V).
Similar content being viewed by others
References
Allport N.L., Cocking T.T.: The colorimetric assay of ergot.Q.J.Pharm. Pharmacol.5, 341 (1932).
Demain A.L.: Cellular and environmental factors affecting the synthesis and excretion of metabolites.J.Appl.Chem.Biotechnol.22, 345 (1972).
Demain A.L., Inamine E.: Biochemistry and regulation of streptomycin and mannosidostreptomycinase (alpha-D-mannosidase) formation.Bacteriol. Rev.34, 1 (1979).
Desai H.G., Desai J.D., Modi V.V.: Regulation of carotenogenesis inPhycomyces blakesleeanus.Ind.J.Microbiol.13, 57 (1973).
Drew S.W., Demain A.L.: Effect of primary metabolites on secondary metabolism.Ann.Rev. Microbiol.31, 343 (1977).
Fiske C.H., Subrarow Y.: The colorimetric determination of phosphorus.J.Biol.Chem.66, 375 (1925).
Hochberg M.L., Sargent M.L.: Separation and identification ofNeurospora phosphatases by polyacrylamide gel electrophoresis.Ann.Biochem.51, 516 (1973).
Krupinski V.M., Robbers J.E., Floss H.G.: Physiological study of ergot: Induction of alkaloid synthesis by tryptophan and its inhibition by phosphate.J.Bacteriol.125, 158 (1976).
Liu C.M., McDaniel L.E., Schaffner C.P.: Factors affecting the production of candicidin.Antimicrob.Agents Chemother.7, 196 (1975).
Mary N.Y., Kelleher W.J., Schwarting A.E.: Production of lysergic acid derivatives in submerged culture. III. Strain selection on defined medium.Lloydia28, 218 (1965).
Petroski R.J., Kelleher W.J.: Biosynthesis of ergot alkaloids. Cell free formation of three products from L-tryptophan and isopentenyl pyrophosphate and their incorporation into lysergic acid amide.Lloydia41, 332 (1978).
Rao K.K., Gupta A.K.: Inhibition of tryptophan synthetase by phosphate in alkaloid producing strain ofAspergillus fumigatus.Naturwiss.62, 395 (1975).
Řeháček Z., Desai J.D., Sajdl P., Pažoutová S.: The cellular role of nitrogen in the biosynthesis of alkaloids by submerged culture ofClaviceps purpurea (Fr.) Tul.Can.J.Microbiol.23, 596 (1977).
Řeháček Z., Sajdl P., Kozová J., Malik K.A., Řičicová A.: Correlation of certain alterations in metabolic activity with alkaloid production by submergedClaviceps.Appl.Microbiol.22, 946 (1971).
Robbers J.E., Robertson L.W., Hornemann K.M., Jindra A., Floss H.G.: Physiological studies on ergot: Further studies on the induction of alkaloid synthesis by tryptophan and its inhibition by phosphate.J.Bacteriol.112, 791 (1972).
Robbers J.E., Eggert W.W., Floss H.G.: Physiological studies on ergot: time factor influence on the inhibitory effect of phosphate and the induction effect of tryptophan on alkaloid production.Lloydia41, 120 (1978).
Sajdl P., Řeháček Z.: Cyclization of chanoclabine-I by cell free preparations from saprophyticClaviceps strain.Folia Microbiol.20, 365 (1975).
Taber W. A.: Fermentative production of hallucinogenic indole compounds.Lloydia30, 39 (1967).
de Waart C.,Taber W.A.: Some aspects of phosphate metabolism ofClaviceps purpurea (Fr.) Tul.Can.J.Microbiol. 6, 675 (1980).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vaidya, H.C., Desai, J.D. Alkaloid production byClaviceps sp. SD-58; Involvement of phosphatase isozymes. Folia Microbiol 28, 12–16 (1983). https://doi.org/10.1007/BF02877378
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02877378