Skip to main content
SpringerLink
Log in

Urease activity inTrichophyton mentagrophytes

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

Urease activity was detected in the dermatophyteTrichophyton mentagrophytes cells at early exponential phase of growth. Specific activity of urease decreased with culture age. At exogenous urea concentrations above 2 mm formation of urease was inhibited. The pH optimum lay at 7–7.5, the Km being 14 mm. No urease activity could be detected in cell-free culture fluid ofT. mentagrophytes. No endoor exocellular urease activity could be detected in aT. rubrum strain grown with or without urea.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ajello L., Georg L.K.:In vitro hair culture for differentiating between atypical isolates ofTrichophyton mentagrophytes andTrichophyton rubrum.Mycopathol. Mycol. Appl. 8, 3 (1957).

    Article  PubMed  CAS  Google Scholar 

  • Bocobo F.C., Benham R.W.: Pigment production in the differentiation ofTrichophyton mentagrophytes andTrichophyton rubrum.Mycologia 41, 291 (1949).

    Article  PubMed  CAS  Google Scholar 

  • Burack A.M., Knight S.G.: Observations on submerged growth and deamination of amino acids by dermatophytes.J. Invest. Derm. 30, 197 (1958).

    PubMed  CAS  Google Scholar 

  • King E.J., Haslewood G.A.D., Delory G.E.: Microchemical methods of blood analysis.Lancet 1, 886 (1937).

    Article  Google Scholar 

  • Littman M.: An improved method for detection of urea hydrolysis by fungi.J.Infect. Dis. 101, 51 (1957).

    PubMed  CAS  Google Scholar 

  • Lowry O.H., Rosebrough N.J., Färr A.L., Randall R.J.: Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265 (1951).

    PubMed  CAS  Google Scholar 

  • Philpot C.: The differentiation ofTrichophyton mentagrophytes fromTrichophyton rubrum by a simple urease test.Sabouraudia 5, 189 (1967).

    PubMed  CAS  Google Scholar 

  • Philpot C.M.: The use of nutritional test for the differentiation of dermatophytes.Sabouraudia 15, 141 (1977).

    PubMed  CAS  Google Scholar 

  • Rosenthal S.A., Sokolsky H.: Enzymatic studies with pathogenic fungi.Dermatol. Internat. 4, 72 (1965).

    Article  CAS  Google Scholar 

  • Silva M., Benham R.W.: Nutritional studies of the dermatophytes with special reference toTrichophyton megnini Blanchard 1896 andTrichophyton gallinae (Megnin 1881) comb. nov.J. Invest. Derm. 18, 453 (1952).

    PubMed  CAS  Google Scholar 

  • Thammayya A., Sanyal M., Basu N.: The relative importance of urease test and hair perforation test in routine differentiation ofTrichophyton rubrum andTrichophyton mentagrophytes.Bull. Cal. School Trop. Med. 16, 43 (1968).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Microbiology Laboratory, Department of Biochemistry, University College of Science, 700 019, Calcutta, India

    A. K. Sanyal & A. B. Banerjee

Authors
  1. A. K. Sanyal
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. B. Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sanyal, A.K., Banerjee, A.B. Urease activity inTrichophyton mentagrophytes . Folia Microbiol 28, 82–86 (1983). https://doi.org/10.1007/BF02877361

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02877361

Keywords

Search

Navigation