Abstract
A modified acridine orange staining method for estimating soil bacterial numbers by epifluorescence microscopy using Synpor filters (VCHZ Synthesia, Czechoslovakia) was elaborated. Comparing with the method of direct count of soil bacteria estimated in a Bürker chamber higher counts of bacteria and a lower variation of results were obtained. To verify the sensitivity of the method, microflora from various soil horizons was tested.
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KrIštůfek, V., Šimek, K., Grunda, B. et al. Use of nitrocellulose Synpor filters for counting soil bacteria by epifluorescence microscopy. Folia Microbiol 32, 349–353 (1987). https://doi.org/10.1007/BF02877225
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DOI: https://doi.org/10.1007/BF02877225