Abstract
A series of experiments were performed withP. aeruginosa to demonstrate which of the biochemical mechanisms are responsible for the resistance to the β-lactam antibiotics. The constitutive β-lactamase was isolated and characterized for the strain used as type OXA whose pI was 7.1, with a molar mass of 49 kg/mol. The strain was also submitted to a series of treatments with Tris and Tris -EDTA to disrupt the outer membrane. The treated cells demonstrated a ten-fold reduction in the MIC with cloxacillin, six-fold with penicillin, and five-fold with oxacillin. At least two different biochemical mechanisms were responsible for the resistance to the β-lactams studied which could be important in the prevalence ofP. aeruginosa in nosocomial infections.
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Soto, U.L., Amadou, S.M., Ramos, F.J. et al. Resistance ofPseudomonas aeruginosa to β-lactam antibiotics. Folia Microbiol 32, 290–296 (1987). https://doi.org/10.1007/BF02877216
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DOI: https://doi.org/10.1007/BF02877216