Summary
The metabolism of glucose in the soil was studied by means of the continuousflow method.
It was found that in the soil used under the given experimental conditions two–thirds to three–fourths glucose added is mineralized to carbon dioxide. The degree of mineralization of glucose added to the medium at the beginning of the experiment or the rate of glucose mineralization in the steady state do not depend on the concentration of glucose, the amount of soil and the presence of mineral forms of nitrogen and phosphorus. the presence of mineral nitrogen and phosphorus compounds affects the rate of glucose mineralization during the first stage of the process.
Continuous feeding of glucose markedly affects the composition of the microbial soil population. When glucose alone is fed continuously the number of bacteria is little affected but that of Azotobacter rises pronouncedly. After a preliminary saturation of the soil with ammonium and phosphate ions or when glucose is fed together with the mineral nutrients a marked rise in the number of bacteria is observed while Azotobacter does not multiphy.
Abstract
С применением непрерывно-проточного метода изучался метаболизм глюкозы в почве.
Было установлено, что в применявшихся образцах почвы при данных условиях опыта от двух третей до трех четвертей прибавляемой глюкозы минерализуются и превращаются в CO2. Ни степень минерализации глюкозы, прибавляемой к почве с начала опыта, ни скорость минерализации глюкозы в постоянном состоянии не зависят ни от концентрации глюкозы, ни от навески почвы, ни от присутствия минеральных форм азота и фосфора. Однако присутствие минеральных соединений азота и фосфора влияет на скорость минерализации гиюкозы в первой стадии процесса.
Непрерывная подача глюкозы выразительно влияет на состав микробного сообщества почвы. При непрерывном протоке раствора одной только глюкозы число бактерий меняется лишь незначительно, но, с другой стороны, существенно повышается количество азотобактера. После предварительного насыщения почвы ионами фосфата аммония, или же при условии одновременной подачи минеральных питательных веществ и раствора глюкозы, наблюдается значительное повышение числа бактерий, тогда как азотобактер не размножается.
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Macura, J., Kunc, F. Continuous flow method in soil microbiology. Folia Microbiol 6, 398–407 (1961). https://doi.org/10.1007/BF02877182
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DOI: https://doi.org/10.1007/BF02877182