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Formation of osmotically fragile rods by the action of lysozyme onBacillus megaterium KM

Возникновение осмотически хрупких палочек под действием лизоцима на Bacillus megaterium KM

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Summary

  1. (1)

    Time-limited incubation ofBacillus megaterium in a medium containing 0.5 M phosphate buffer and 0.2 mg. lysozyme/ml. results in the formation of osmotically fragile cells.

  2. (2)

    The osmotically fragile cells, in common with protoplasts, are Gram-negative but retain their rod shape.

  3. (3)

    When the stabilized medium is diluted with distilled water the osmotically fragile cells are broken up and cytoplasmic proteins are solubilized. Protein solubilization is increased by freezing and thawing of the osmotically fragile cells.

  4. (4)

    A part of the osmotically fragile cells are able to regenerate on peptone agar with 10% sucrose. The regenerated cells are capable of growth on broth and on nutrient agar without stabilizers.

  5. (5)

    The osmotically fragile cells and ghosts derived from them will incorporate14C-diaminopimelic acid which represents a component of the cell wall. This incorporation and the radioactivity of the TCA-insoluble fraction cannot be solubilized with trypsin.

Abstract

  1. (1)

    В течение непродолзительной инкубации Bacillus megaterium в среде с 0,5 M фосфатным буфером и лизоцимом (0,2 мг/мл) вознпкают осмотически хрупкие клетки.

  2. (2)

    Эти осмотически хрупкие клетки, как и протопласты, оказываются грамотрицательными и сохраняют палочкообразную форму.

  3. (3)

    Разведение стабилизированной среды дистиллированной водой вызывает разрывы этих палочек и растворение цитоплазматических белков. Возможно усилить растворение белков, замораживая и оттаивая осмотически хрупкие клетки.

  4. (4)

    Часть осмотически хрупких клеток способна к регенерации на пептонном агаре с 10% сахарозы. Регенерировавшие клетки способны расти на бульоне и мясопептонном агаре без стабилизаторов.

  5. (5)

    Осмотически хрупкие клетки и возникшие из них ghosts включают C14-диаминопимельную кислоту, которая является составной частью клеточной стенки. Это включение не чувствительно к хлорамфениколу, и радиоактивность невозможно растворить из нерастворимой в трихло руксусной кислоте фракции трипсином.

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Authors and Affiliations

  1. Department of Microbiology and Isotope Laboratory, Institute of Biology, Czechoslovak Academy of Sciences, Prague 6

    J. Chaloupka & K. Vereš

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  1. J. Chaloupka
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  2. K. Vereš
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Chaloupka, J., Vereš, K. Formation of osmotically fragile rods by the action of lysozyme onBacillus megaterium KM. Folia Microbiol 6, 379–385 (1961). https://doi.org/10.1007/BF02877179

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  • DOI: https://doi.org/10.1007/BF02877179

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