Abstract
Escherichia coli B bacteria cultivated under aerobic and anaerobic conditions were irradiated with X-rays at different phases of growth of the culture (at the outset and end of the logarithmic phase and in the stationary phase). Changes in the nucleic acid and protein content and in the number of nuclear equivalents per cell were determined in irradiated bacteria. The extrapolation number of the survival curves increased proportionately to the increase in the cell components. It was not in direct agreement with the increase in any of the individual components, however. The slope of the survival curves changed simultaneously with the extrapolation number, showing that radiosensitivity changes were probably of a complex character and that they might be the function of at least two factors, manifested as a change in the number and size of the targets (evaluated from the aspect of the target theory).
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References
Billen, D.:Alterations in the radiosensitivity of Escherichia coli through modification of cellular macromolecular components. Biochim. biophys. Acta 34: 110, 1959.
Burton, K.:A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J. 62: 315, 1956.
Ceriotti, G.:Determination of nucleic acids in animal tissues. J. biol. Chem. 214: 59, 1955.
Delbrück, M., Luria, S. E.:Interference between two bacterial viruses acting upon the same host and the mechanism of virus growth. Arch. Biochem. 1: 111, 1942.
Herčík, F.:From the atom to life. Praha 1946.
Hollaender, A., Stapleton, G. E., Martin, F. L.:X-ray sensitivity of E. coli as modified by oxygen tension. Nature 167: 103, 1951.
Kaplan, H. S., Moses, L. E.:Biological complexity and radiosensitivity. Science 145: 21, 1964.
Lowry, H. O., Rosebrough, N. J., Farr, A. L., Randall, R. J.:Protein measurement with the folin phenol reagent. J. biol. Chem. 193: 265, 1951.
Moiseenko, E. V.:Nucleic acid content and radioresistance in Escherichia coli. Biophyzika 5: 176.
Myslivec, V.:Statistical methods in agricultural and forestry research. Praha 1957.
Powers, E. L.:Considerations of survival curves and target theory. Phys. Med. Biol. 7: 3. 1962.
Roth, Z., Josífko, M., Malý, V., Trčka, V.:Statistical methods in experimental medicine. Praha 1962.
Sargent, T.:Effects of pH and anoxia on the cell morphology and X-ray sensitivity of Escherichia coli. Rad. Res. 14: 323, 1961.
Stapleton, G. E.:Variations in the sensitivity of E. coli to ionizing radiations during the growth cycle. J. Bacteriol. 70: 357, 1955.
Ševčík, F., Liška, B., Hošek, B.:Apparatus for automatic inoculation and recording of growth curves of bacteria. Fol. microbiol. 8: 125, 1964.
Vinter, V.:Spores of microorganisms. VIII. The synthesis of specific calcium and cystine-containing structures in sporulating cells of Bacilli. Fol. microbiol. 5: 217, 1960.
Zimmer, K. G.:The problems of quantitative radiobiology Moskva 1962.
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Vízdalová, M., Liška, B. Radiosensitivity ofEscherichia coli B bacteria containing different amounts of nucleic acids and proteins. Folia Microbiol 11, 21–28 (1966). https://doi.org/10.1007/BF02877151
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DOI: https://doi.org/10.1007/BF02877151