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Antibiotic-induced changes of mycelial growth ofBotrytis cinerea

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Abstract

The effect of antibiotics and metabolic inhibitors on mycelial growth ofBotrytis cinerea was followed. Inhibitors of protein synthesis, chloramphenicol, erythromycin and tetracycline inhibit growth or sporulation ofBotrytis cinerea. Ethidium bromide, 5-fluorouracil, phenylethylalcohol and K 20 cause granulation, vacuolization and undulation of hyphae. 2,4-Dinitrophenol, boromycin, macrotetrolides, monensin, scopathricin and TX2 at subfungistatic concentrations induce intensive branching of hyphal tipsi.e. at the site of synthesis of the cell wall. In older hyphae grown in the absence of the antibiotics the branching begins after their addition, particularly in the septum region. When comparing the results referred to here with those obtained previously and on the basis of literature data it may be assumed that the changes in polarity of growth ofBotrytis cinerea might be caused primarily or secondarily by impairing membrane functions and formation of cell walls.

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References

  • Banerjee S., Margulis L.: Reversible inhibition of cilia regeneration inStentor coeruleua by isopropyl-n-phenyl carbamate.Nature, (London) 224, 180 (1969).

    Article  CAS  Google Scholar 

  • Banerjee S., Margulis L.: Inhibition of cilia regeneration by neoplastic agents.Cancer Chemotherapy Reports 55, 531 (1971).

    CAS  Google Scholar 

  • Baráth Z., Babáthová H., Betina V., Nemec P.: Production and release of riboflavin from the myceliumEremothecium ashbyi in the presence of cyanein and amphotericin B. (In Slovak)Biológia (Bratislava) 24, 684 (1969).

    Google Scholar 

  • Baráth Z., Babáthová H., Betina V., Nemec P.: Ramihyphins-antifungal and morphogenetic antibiotics fromFusarium sp. S-435.Folia Microbiol. 19, 507 (1974).

    Google Scholar 

  • Baráthová H., Betina V., Nemec P.: Morphological changes induced in fungi by antibiotics.Folia Microbiol. 14, 475 (1969).

    Google Scholar 

  • Baráthová H., Betina V., Baráth Z., Nemec P.: Morphogenic effects of ramihyphin A in filamentous fungi.Folia Microbiol. 20, 97 (1975).

    Article  Google Scholar 

  • Betina V., Babáthová H.: Citrinin-an inducer of permeability changes inEremothecium ashbyi.J. Antibiot. Ser. A 21, 628 (1968).

    Google Scholar 

  • Betina V., Mičeková D.: Antimicrobial properties of fungal macrolide antibiotics.Zeitsch. Allg. Mikrobiol. 12, 355 (1972).

    Article  CAS  Google Scholar 

  • Betina V., Rúčková E.: Effect of citrinin, desertomycin and of polyene antibiotics on plant and animal cell membranes.Biológia (Bratislava) 27, 463 (1972).

    CAS  Google Scholar 

  • Betina V., Mičeková D.: Morphogenetio activity of cytochalasins, cyanein and monorden inBotrytis cinerea.Zeitsch. Allg. Mikrobiol. 13, 287 (1973).

    Article  CAS  Google Scholar 

  • Betina V., Droubet E., Segretain G.: Action de la cyanéine invitro sur des champignons pathogènes.Ann. Inst. Pasteur 109, 933 (1965).

    CAS  Google Scholar 

  • Betina V., Betinová M., Kutková M.: Effects of cyanein on growth and morphology of pathogenic fungi.Arch. Mikrobiol. 55, 1 (1966).

    Article  PubMed  CAS  Google Scholar 

  • Betina V., Baráthová H., Nemec P., Baráth Z.: Permeability changes caused by desertomycin in fungal, animal and plant cells.J. Antibiot. 22, 129 (1969).

    PubMed  CAS  Google Scholar 

  • Betina V., Janstová D., Spišiaková J.: Effect of antibiotics on the life cycle ofNeurospora crassa.Folia Microbiol. 20, 340 (1975).

    CAS  Google Scholar 

  • Betina V., Mičeková D., Nemec P.: Antimicrobial properties of cytochalasina and their alteration of fungal morphologyJ. Gen. Microbiol. 71, 343 (1972).

    CAS  Google Scholar 

  • Brian P. W., Curtis P. J., Hemming H. G.: A substance causing abnormal development of fungal hyphae produced byPenicillium janczewskii.Trans. Brit. Mycol. Soc. 29, 173 (1946).

    Article  CAS  Google Scholar 

  • Esuruoso O. F., Price T. V., Wood R. K. S.: Germination ofBotrytis cinerea conidia in the presence of quintozene, teonazine and dicloran.Trans. Brit. Mycol. Soc. 51, 405 (1968).

    CAS  Google Scholar 

  • Fuska J., Kuhr I., Nemec P., Fusková A.: Antitumor antibiotic produced byPenicillium stipitatum Thorn.J. Antibiot. 27, 123 (1974).

    PubMed  CAS  Google Scholar 

  • Fuska J., Sláviková E., Adámková M.: Secondary metabolites ofP. stipitatum Thom. I. Compounds of tropolone character. (In Slovak)Biológia (Bratislava),30, 669 (1975).

    CAS  Google Scholar 

  • Gale E. F., Cundliffe E., Reynolds P. E., Richmond M. H., Waking M. J.: The molecular basis of antibiotic action. John Wiley and Sons, London, pp. 147, 151 (1972).

    Google Scholar 

  • Henderson P. J. F., Lardy H. A.: Bongkrekic acid. An inhibitor of the adenine nucleotic translocase of mitochondria.J. Biol. Ghem. 245, 1319 (1970).

    CAS  Google Scholar 

  • Holík M., Kuhr I.: Determination of structure of stipitalide: a new tropolone fromPenicillium stipitatum Thom.Ghem. Commun. 1973, 65 (1973)

    Article  Google Scholar 

  • Huber F. M.:Griseofulin. In: Gottlieb, D., Shaw P. D. (Editors), Antibiotics, Volume I, Mechanism of Action. Springer-Verlag, Berlin, p. 181–189 (1967).

    Google Scholar 

  • Hütter R., Keller-Schierlein W., Nüesch J., Zähner H.: Scopamycine.Arch. Mikrobiol. 51, 1 (1965).

    Article  Google Scholar 

  • Iwasa I., Higashide E., Yamamoto H., Shibata M.: Studies on validamycins, new antibiotics. II. Production and biological properties of validamycins A and B.J. Antibiot. 24, 107 (1971).

    PubMed  CAS  Google Scholar 

  • Katz D., Rosenberg R. F.: Hyphal wall synthesis inAspergillus nidulans: effect of protein synthesis inhibition and osmotic shock on chitin insertion and morphogenesis.J. Bacteriol. 108, 184 (1971).

    PubMed  CAS  Google Scholar 

  • Katz D., Goldstein D., Rosenberger R. F.: Model for branch initiation inAspergillus nidulans based on measurements of growth parameters.J. Bacteriol. 109, 1097 (1972).

    PubMed  CAS  Google Scholar 

  • Lysek G.: Zonierungen und Hexenringe Morphologische Differezierungen bei Pilzen.Naturwiss. Rundschau 27, 449 (1974).

    Google Scholar 

  • Malawista S. E., Sato H., Bensch K. G.: Vinblastine and griseofulvin reversibly disrupt the living mitotic spindle.Science (Washington) 160, 770 (1968).

    Article  CAS  Google Scholar 

  • Mizel S. B., Wilson L.: Inhibition of the transport of several hexoses in mammalian cells by cytochalasinJ. Bid. Ghem. 247, 4102 (1972).

    CAS  Google Scholar 

  • Nioh T., Mizushima S.: Effect of validamycin on the growth and morphology ofPellicularia sasakii.J. Gen. Appl. Microbiol. 20, 373 (1974).

    Article  CAS  Google Scholar 

  • Oliver P. T. P.: Influence of cytochalasin B on hvphal morphogenesis ofAspergillus nidulans.Protoplasma 76, 279 (1973).

    Article  PubMed  CAS  Google Scholar 

  • Pache W.:Boromycin. In: Corcoran J. W., Hahn F. E.: Antibiotics III. Berlin-Heidelberg-New York, Springer, p. 585 (1975).

    Google Scholar 

  • Pache W., Zähner H.: Studies on the mechanism of action of boromycin.Arch. Mikrobiol. 67, 156 (1969).

    Article  PubMed  CAS  Google Scholar 

  • Richmond D. V., Pring R. J.: The effect of benomyl on the fine structure ofBotrytis foboe.J. Gen. Microbiol. 66, 79 (1971).

    CAS  Google Scholar 

  • Šašek V., Musílek V.: Effects of the new antifungal antibiotic mucidin II. Morphological changes brought about in sensitive test microorganisms.Folia Microbiol. 19, 142 (1974).

    Article  Google Scholar 

  • Šubík J., Behúň M.: Effect of bongkrekic acid on growth and metabolism of filamentous fungi.Arch. Microbiol. 97, 81 (1974).

    Article  Google Scholar 

  • Šubík J., Kolarov J., Kováč L.: Obligatory requirement of mitochondrial ATP for normal functioning of the eucaryotic cell.Biochem. Biophys. Res. Commun. 49, 192 (1972).

    Article  PubMed  Google Scholar 

  • Šubík J., Behúň M., Šmigáň P. Musílek V.: Mode of action of mucidin a new antifungal antibiotic produced by the basidiomyceteOudemansiella mucida.Biochim. Biophys. Acta 343, 363 (1974).

    PubMed  Google Scholar 

  • Wessels N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamadak M.: Microfilaments, in cellular and development processes.Science, (Washington)17, 135 (1971).

    Article  Google Scholar 

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  1. Department of Technical Microbiology and Biochemistry, Faculty of Chemistry, Slovak Polytechnical University, 880 37, Bratislava

    H. Baráthová & V. Betina

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  1. H. Baráthová
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  2. V. Betina
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Baráthová, H., Betina, V. Antibiotic-induced changes of mycelial growth ofBotrytis cinerea . Folia Microbiol 21, 355–361 (1976). https://doi.org/10.1007/BF02876961

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