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The wild strainStreptomyces coeruleorubidus JA 10092 was found to segregate into two spontaneous morphological variants (spo-1 andbld-1) with a different ability to form aerial mycelium in media with glucose as the main carbon source. Six new types of developmental mutants were obtained from the bald variantbld-1 after treatment with mutagens (UV light, γ radiation, nitrous acid) and after natural selection Formation of the aerial mycelium was fully suppressed in thebld-2 type growing on media both with glucose and with starch. The other types were bald only on starch media, forming the aerial mycelium on media with glucose; typesspo-2, spo-3, spo-4 andspo-5 differed in size, shape and surface structure of spores, the typewhi formed asporogenous aerial hyphae.
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Blumaueroá M., Lipavská H., Stajner K., Vaněk Z.: The study of variability and strain selection inStreptomyces atroolwaceus. III. Isolation and preliminary characteristic of mutants impaired in the biosynthesis of mithramycin.Folia Microbiol,21, 285 (1976)
Blumauerová M., Matějů J., Stajner K., Vaněk Z.. Studies on the production of daunomycinonederived glycosides and related metabolites inStreptomyces coeruleorubidus andStreptomyces peucetius.Folia Microbiol. 22, 275 (1977).
Blumauerová M., Pokorný V., Šťastná J., Hošťálek Z., Vaněk Z.: Improved yields of daunomycinone glycosides m developmental mutants ofStreptomyces coeruleorubidus.Folia Microbiol. 23, in press (1978a).
Blumauerová M., Stajner K., Pokorný V., Hošťálek Z., Vaněk Z. Mutauts ofStreptomyces coeruleorubidus impaired in the biosynthesis of daunomycmone glycosides and related metabolites.Folia Microbiol. 23, in press (1978b).
Brazhnikova M. G., Konstantinova N. V., Pomazkova V. A., Zakharov B. V.: Physicochemical properties of antitumour antibiotic rubomycin, produced byActinomyces coeruleorubidus. (In Russian)Antibiotiki 11, 763 (1966).
Chater K. F.: A morphological and genetic mapping study of white colony mutants ofStreptomyces coelicolor.J. Gen. Microbiol. 72, 9 (1972).
Chater K. F., Merrick M. J.: Approaches to the study of differentiation inStreptomyces coelicolor A 3(2). Second International Symposium on Genetics of Industrial Microorganisms (K. D. Macdonald, Ed.), p. 583. Academic Press, London-New York-San Francisco (1976).
Hesseltine C. W., Benedict R. G., Pridham F. G.: Useful criteria for species differentiation in the genusStreptomyces.Ann. N.Y. Acad. Sci. 60, 136 (1954).
Hopwood D. A., Chater K. F., Dowding J. E., Vivian A.: Advances inStreptomycea coelicolor genetics.Bacteriol. Rev. 37, 371 (1973).
HopWood D. A., Sermonti G.: The genetics ofStreptomyces coelicolor.Adv. Genet. 11, 273 (1962).
HopWood D. A., Wildermuth H., Palmer H. M.: Mutansof Streptomyces coelicolor defective m sporulationJ. Gen. Microbiol. 61, 397 (1970).
Kalakoutskii L. V., Agre N. S.: Comparative aspects of development and differentiation inActinomycetes.Bacteriol. Rev. 40, 469 (1976).
Lechevalier H. A., Tikhonenko A. S.: Effect of nutritional conditions on the spore surface ofActinomycetes. (In Russian)Mikrobiologiya 29, 43 (1960).
Merrick M. J.: A morphological and genetic mapping study of bald colony mutants ofStreptomyces coelicolor.J. Gen. Microbiol. 96, 299 (1976).
Preobrazhenskaya T. P., Manaphova N. A., Gauze G. F.: Systematic position, variation and antibiotic properties of rubomycin producing organism. (In Russian)Antibiotiki 11, 867 (1966).
Redshaw P. A., McCann P. A., Sankaran L., Pogell B. M.: Control of differentiation in Streptomycetes: Involvement of extrachromosomal deoxyribonucleic acid and glucose repression m aerial mycelia development.J. Bactenol. 125, 698 (1976).
Saburova T. P., Lapchinskaya O. A., Kruglyak E. B.: Selection ofActinomyces coeruleorubidus, rubidomycin-producing organism. (In Russian)Antibiotiki 19, 509 (1974).
Shirling E. B., Gottlieb D.: Cooperative description of type cultures ofStreptomyces. III. Additional species description from first and second studies.Internat. J. Syst. Bactenol. 18, 279 (1968).
Rhône-Poulenc S. A.: Nouvel antibiotique et sa préparation.Belgian Pat. 632,391 (1963).
Rhône-Poulenc S. A.: Nouvel antibiotique et sa préparation par culture d’un streptomyce.French Pat. 1,583,752 (1969)
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Blumauerová, M., Pokorný, V., Šťastná, J. et al. Developmental mutants ofStreptomyces coeruleorubidus, a producer of anthracyclines: Isolation and preliminary characterization. Folia Microbiol 23, 177–182 (1978). https://doi.org/10.1007/BF02876576
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DOI: https://doi.org/10.1007/BF02876576