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Isolation of glucosyltransferase fromStreptomyces aureofaciens

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Abstract

Streptomyces aureofaciens B 96 grown on a synthetic medium glucosylated exogenous 1,2-dihydroxy-9, 10-anthraquinone (alizarin). The glucosylation was inhibited by 2,4-dinitrophenol added to the cultivation medium. A cell-free preparation was obtained from the mycelium isolated after 16 h of growth and was found to catalyze the transfer of glucose from glucosyluridyl diphosphate to 1,2-dihydroxy-9, 10-anthraquinone, giving rise to l-hydroxy-2-(β-D-glueopyranosyloxy)-9,10-anthraquinone.

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Matějů, J., Cudlín, J., Steinerová, N. et al. Isolation of glucosyltransferase fromStreptomyces aureofaciens . Folia Microbiol 23, 337–340 (1978). https://doi.org/10.1007/BF02876431

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