Abstract
Streptomyces aureofaciens B 96 grown on a synthetic medium glucosylated exogenous 1,2-dihydroxy-9, 10-anthraquinone (alizarin). The glucosylation was inhibited by 2,4-dinitrophenol added to the cultivation medium. A cell-free preparation was obtained from the mycelium isolated after 16 h of growth and was found to catalyze the transfer of glucose from glucosyluridyl diphosphate to 1,2-dihydroxy-9, 10-anthraquinone, giving rise to l-hydroxy-2-(β-D-glueopyranosyloxy)-9,10-anthraquinone.
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Blumauerova’ M., Mráček M., Vondráčková J., Podojil M., Hošťálek Z., Vaněk Z.: Regulation of biosynthesis of secondary metabolites. IX. The biosynthetic activity of blocked mutants ofStreptomyces aureofaciens.Folia Microbiol. 14, 215 (1969).
Cudlín J., Vaněk Z., Hovorková N., Blumauerová M., Vondráček M., Benda A.: Biosynthetic production of quinone glucosides. Czech, pat.144 539 (1971).
Cudlín J., Steinerová N., Matějů J., Blumauerová M., Vaněk Z.: Microbial transformation of 1,8-dihydroxy-9,10-anthraquinone.Coll. Czech. Chem. Commun. 43, 1803 (1978).
Hopkinson S. M.: The chemistry and biochemistry of phenolic glycosides.Quart. Rev. 23, 98 (1969).
Hovorková N., Cudlín J., Matějů J., Blumauerová M., Vaněk Z.: Microbial glucosidation of alizarin and anthraflavin.Coll. Czech. Chem. Commun. 39, 662 (1974a).
Hovorková N., Cudlín J., Matějů J., Blumauerová M., Vaněk Z.: Microbial glucosidation of monohydroxyanthraquinones.Coll. Czech. Chem. Commun. 39, 3568 (1974b).
Kelleher W. J.: Glycoside biosynthesis and the biochemistry of sugar nucleotides.J. Pharm. Sci. 54, 1081 (1965).
Kochetkov N. K., Shibaev V. N.: Glycosyl esters of nucleotide pyrophosphates.Adv. Carb. Chem. 28, 307 (1973).
Lowry O. H., Rosenbrough N. J., Farr A. L., Randall R. J.: Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193, 265 (1951).
Matějů J., Cudlín J., Hovorková N., Blumauerová M., Vaněk Z.: Microbial glucosidation of dihydroxyanthraquinones. General properties of the glucosidation system.Folia Microbiol. 19, 307 (1974).
Nikaido H., Hassid W. Z.: Biosynthesis of saccharides from glucopyranosyl esters of nucleotide pyrophosphates (”Sugar nucleotides“).Adv. Carb. Chem. 26, 351 (1971).
Sato M., Hasegawa M.: A transglucosylase inDaphne odora converting daphnin to daphnetin 8-glucoside.Phytochemistry 10, 2367 (1971).
Vokoun J., Vaněk Z., Podojil M., Blumauerová M., Vondráček M., Benda A.: Czech. Pat.153 722 (1974).
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Matějů, J., Cudlín, J., Steinerová, N. et al. Isolation of glucosyltransferase fromStreptomyces aureofaciens . Folia Microbiol 23, 337–340 (1978). https://doi.org/10.1007/BF02876431
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DOI: https://doi.org/10.1007/BF02876431