Abstract
7 days or 7 weeks old alfalfa plants (Medicago sativa L.), susceptible (S) and resistant (R) to bacterial wilt, were inoculated withCorynebacterium michiganense pv.insidiosum and on day 8 and 15 after inoculation the levels of acid-soluble phosphate esters (P-esters) were determinated by means of32P labelling in the shoots or roots. The most significant changes were recorded in the roots of the older R plants grown in full Knop nutrient solutions on day 8 after inoculation. The marked reduction of inorganic phosphate (P1) uptake by whole R plants is accompanied by a decrease in the levels of fructose-l, 6-bisphosphate (Fru-P2), glucose-6-phosphate (Glc-6-P), fructose-6-phosphate (Fru-6-P), adenosine mono-, and diphosphate (AMP and ADP), phosphorylcholine (P-choline) and a proportional increase in the level of P1. In the S plants, infection affected neither P1 uptake nor P1 proportions. In the plants grown after inoculation in diluted Knop’s solutions (0.147 mM KH2PO4), infection induced a reduction of the radial transport of P1 to the segments of R roots whereas a reduction of the levels was only recorded in some P-esters [AMP, ADP, dihydroxyacetone phosphate (DHAP), and P-choline, but no decrease of Fru-P2, Glc-6-P and Fru-6-P]. In the S plants, P1 transport and the levels of P-esters were increased by the infection. P1 transport exhibited considerable metabolic dependence (DNP, DCCD). Bacterial infection probably had no influence on the activity of the plasma membrane ATPases.
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Abbreviations
- AMP, ADP, ATP:
-
adenosine mono-, di-, or triphosphate
- IJCCD:
-
N, N′-dicyclohexylcarbodiimide
- DHAP:
-
dihydroxyacetone phosphate
- DNP:
-
2,4-dinitro- phenol
- Fru-P2 :
-
fructose-1,6-bisphosphate
- Fru-6-P2 :
-
fructose-6-phosphate
- Glc-6-P:
-
glucose-6-phosphate
- P1 :
-
inorganic orthophosphate
- P-choline:
-
phosphoryicholine
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Hanker, I., Kůdelová, A. The bacterial wilt, uptake of phosphate, and phosphate ester levels in the resistant and susceptible alfalfa plants. Biol Plant 30, 63–73 (1988). https://doi.org/10.1007/BF02876428
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DOI: https://doi.org/10.1007/BF02876428