Abstract
The synthesis of peptides by manual solid-phase methods requiring very small volume of solvents at different steps is described. The syntheses have been carried out on polystyrene-based resins using fluorenylmethoxy carboxyl chemistry. All reactions were carried out in vials, of capacity 1.2 or 6 ml, with gentle stirring. The volumes of solvents used were 1–2 ml for each operation. The crude peptides after cleavage from the resin were reasonably pure. The total volume of solvents required for the entire synthesis is substantially less as compared to volumes required in semi-automated and fully automated peptide synthesizers which renders the described method cost-effective.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Atherton E and Sheppard R C 1989 InSolid phase peptide synthesis: A practical approach (Oxford: IRL)
Barany G and Merrifield R B 1980 InThe peptides: Analysis, synthesis biology (eds) I Gross and J Meienhofer (New York: Academic Press) vol. 2 pp 3–285
Blondelle S E, Perez-Paya E, Dooley C T, Pinilla C and Houghten R A 1995Trends Anal. Chem. 14 83
Choma C T, Lear J P, Nelson M J, Dutton R L, Robertson D E and DeGrado W F 1994J. Am. Chem. Soc. 116 856
Eichler J and Houghten R A 1995Mol. Med. To-day 1 174
Erickson S W and Merrifield R B 1976 InThe proteins (eds) H Neurath and R L Hills (New York: Academic Press) vol. 11, pp. 257–528
Finn F M and Hofmann K 1976 InThe proteins (eds) H Neurath and R L Hills (New York: Academic Press) vol. 11, pp. 2–105
Fodor S P A, Leighton Read J, Pirrung M L, Stryer L, Tsai Lu A and Solas D 1991Science 251 767
Kent S B H 1988Ann. Rev. Biochem. 57 957
Liu C F, Rao C and Tam J P 1996J. Am. Chem. Soc. 118 307
Merrifield R B 1986Science 232 341
Millon de L R C, Milton S C F and Kent S B H 1992Science 256 1445
Sitaram N, Chandy M, Pillai V N R and Nagaraj R 1992Antimicrob. Agents Chemother. 36 2468
Sitaram N, Subbalakshmi C and Nagaraj R 1995Int. J. Pept. Protein Res. 46 166
Wlodawer A, Miller M, Jaskolski M, Sathyanarayana B K, Baldwin E, Weber I T, Selk L M, Clawson L, Schneider J and Kent S B H 1989Science 245 616
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bikshapathy, E., Nagaraj, R. Manual solid-phase syntheses of peptides on resins with high loading capacity requiring small volumes of solvents. Proc. Indian Acad. Sci. (Chem. Sci.) 109, 319–323 (1997). https://doi.org/10.1007/BF02875973
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02875973