Abstract
The ability of certain substances to activate lymphocytes in terms of the production of biologically active substances was studied. These substances were tested by following their migration inhibitory activity. The capacity of concanavalin A and commercial preparations of phytohaemagglutinin (PHA-M and PHA-P) to induce formation of MIF was confirmed. In addition, similar activities were found even in antirabbit antilymphocyte serum (ALS) and erythrogenic toxin (ET). The production of MIF was usually found in material obtained from rabbits treated with complete Freund adjuvant (CFA). On the other hand, material obtained from non-treated rabbits, was found to be inactive with the exception of thymus from young rabbits. The results support the significance of an increasing pharmacological potency of cells in the course of sensitization with CFA. The evidence concerning the release of MIF by ET was extended by similar finding in rabbits made tolerant to the pyrogenic activity of this particular toxin. Lymphocytes obtained from rabbits that were unable to respond to ET by fever, could not liberate MIF (at the same time) upon incubation with ET, even though these lymphocytes produced MIF normally after incubation with PPD tuberculin or concanavalin A. The importance of these results is discussed in terms of the immunologically nonspecific activation of lymphocytes that can mimic immunologically specific events.
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Pekárek, J., Švejcar, J., Hříbalová, V. et al. The production of migration inhibitory factor (MIF) in rabbit lymphocytes by other than immunological mechanism. Folia Microbiol 18, 393–401 (1973). https://doi.org/10.1007/BF02875935
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DOI: https://doi.org/10.1007/BF02875935