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Histone-induced changes of protein synthesis inEscherichia coli

II. Effect of histone on alkaline phosphatase synthesis

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Abstract

The addition of histone to the medium in the logarithmic phase of growth ofEscherichia coli influences alkaline phosphatase synthesis in two ways: it delays initiation of derepression of synthesis of the enzyme by about 60–80 min and it inhibits its synthesis. The inhibitory effect persists even after removing histone from the medium. In stationary-phase of growth the inhibitory effect of histone is obliterated. From an analysis of the initial kinetics of derepressed alkaline phosphatase synthesis and from previous results (Štrbáňová-Nečinováet al., 1972) it is concluded that histone added toEscherichia coli cells interferes with the synthesis of mRNA for alkaline phosphatase.

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  1. S. Štrbáňová-Nečinová

    Present address: Department of Medical Microbiology and Immunology, Prague 2

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  1. Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4

    E. Masnerová & S. Štrbáňová-Nečinová

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  1. E. Masnerová
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  2. S. Štrbáňová-Nečinová
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Part I: Folia Microbiol.17, 170 (1972).

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Masnerová, E., Štrbáňová-Nečinová, S. Histone-induced changes of protein synthesis inEscherichia coli . Folia Microbiol 18, 368–375 (1973). https://doi.org/10.1007/BF02875931

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