Abstract
Thymineless death (TLD) as well as deoxyribosideless death (DRLD) can be observed inLactobacillus acidophilus R-26 during growth in media lacking thymine or deoxyriboside respectively. Both phenomena exhibit the same interval of lage period (2–3 h) but the rate of inactivation is 2–3 times faster in TLD. Transfer experiments show that inactivation of bacterial reproduction is accelerated immediately if—DR medium is replaced by—T one. In the opposite case the deceleration of the inactivation rate does not appear immediately but after a 1–2 h lag period, in which no changes in the number of viable bacteria can be observed. Our results suggested that the accumulation of deoxyriboside compounds has no causal role in the inactivation of bacterial reproduction. However, the presence of deoxyribosides can accelerate the process of inactivation.
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References
Cohen S. S., Barner H. D.: Studies on unbalanced growth inEscherichia coli.Proc. Nat. Acad. Sci. U.S. 40, 885 (1954).
Cummings D. J., Taylor A. L.: Thymineless death and its relation to UV sensitivity inEscherichia coli.Proc. Nat. Acad. Sci. U.S. 56, 171 (1966).
Cummings D. J., Mondale L.: Thymineless death inEscherichia coli: Strain specificity.J. Bacteriol. 93, 1917 (1967).
Freifelder D.: Single-strand breaks in bacterial DNA associated with thymine starvation.J. Mol. Biol. 45, 1 (1969).
Freifelder D., Katz G.: Persistence of small fragments of newly synthetized DNA in bacteria following thymidine starvation.J. Mol. Biol. 57, 351 (1971).
Goldthwait D., Jacob F.: Sur le mécanisme de l'induction du développement du prophage chez les bactéries lysogènes.Compt. Rend. Acad. Sci. Paris 259, 661 (1964).
Hertman I., Luria S. E.: Transduction studies on the role of arec + gene in the ultraviolet induction of prophage lambda.J. Mol. Biol. 23, 117 (1967).
Korn D., Weissbach A.: Thymineless induction inEscherichia coli K12 (lambda).Biochem. Biophys. Acta 61, 775 (1962).
Melechen N. E., Skaar P. D.: The provocation of an early step of induction by thymine deprivation.Virology 16, 21 (1962).
Nakayama H., Hanawalt P.: Integrity of DNA in thymine-starvedEscherichia coli.Fed. Proc.,31, 1168 (1972)
Neuhard J.: Studies of the acid-soluble nucleotide pool in thymine-requiring mutants ofEscherichia coli during thymine starvation. III. On the regulation of the de-adenosine-5′-triphosphate and de-cytidine-5′-triphosphate pools ofEscherichia coli.Biochem. Biophys. Acta 129, 104 (1966).
Neuhard J., Thomassen E.: Turnover of deoxyribonucleosides triphosphates inEscherichia coli 15T-during thymine starvation.Europ. J. Biochem. 20, 36 (1971).
Pauling C., Hanawalt P.: Nonconservative DNA replication in bacteria after thymine starvation. Proc.Nat. Acad. Sci. U.S. 54, 1728 (1965).
Reich J., Soška J.: The thymineless death and deoxyribosideless death inLactobacillus acidophilus R-26.Biochem. Biophys. Res. Commun. 29, 62 (1967).
Reich J., Soška J.: Thymineless death inLactobacillus acidophilus R-26.Folia Microbiol. 15, 40 (1970).
Reiter H., Ramareddy G.: Loss of DNA behind the growing point of thymine starvedBacillus subtilis 168.J. Mol. Biol. 50, 533 (1970).
Soška J.: Growth ofLactobacillus acidophilus in the absence of folic acid.J. Bacteriol. 91, 1840 (1966).
Soška J., Lark K. G.: Regulation of nucleic acid synthesis inLactobacillus acidophilus R-26.Biochim. Biophys. Acta 119, 526 (1966).
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Reich, J., Soška, J. Thymineless death inLactobacillus acidophilus R-26. Folia Microbiol 18, 361–367 (1973). https://doi.org/10.1007/BF02875930
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DOI: https://doi.org/10.1007/BF02875930