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Synchronized growth ofMycobacterium phlei

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Abstract

Three synchronization methods, cold shock, starvation, centrifugation and their combinations were used in order to induce synchronized division inMycobacterium phlei PA. It was found that a combination of cold shock with a preceding centrifugation is the optimum method for the synchronized growth of the culture, its use resulting in three synchronized cell divisions.

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Authors and Affiliations

  1. Department of Bacterial Genetics, Institute of Microbiology, Ozechoslovak Academy of Sciences, Prague 4

    J. Koníček

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  1. J. Koníček
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Koníček, J. Synchronized growth ofMycobacterium phlei . Folia Microbiol 17, 192–195 (1972). https://doi.org/10.1007/BF02875813

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  • DOI: https://doi.org/10.1007/BF02875813

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