Abstract
A new polarographic immunoassay was developed. In this assay, human serum albumin (HSA) as the model antigen was covalently labeled with organic compound erythrosin B (EB) containing four non-radioactive iodides through 1-step chemical reaction. The labeling procedure is simple and the conditions needed are moderate. The molar laheling ratlo of EB: HSA was 12: 1. The content of iodine in the conjugate obtained by the proposed procedure is much higher than that by the other existing methods. A heterogeneous competitive immunoassay was established by coupling the catalysis of the conjugate to substrate As (III) -Ce (IV) reaction with the linear-sweep polarographic detection of As (III) amount. HSA can be determined in the HSA concentration range from 1 to 200 μg/mL, with the detection limit of 0. 66,μg/mL.
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Project supported by the National Natural Science Foundation of China
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Song, J., Bai, Y., Guo, W. et al. Polarographic immunoassay coupled with catalysis of non-radioactive multiple iodine label. Sc. China Ser. B-Chem. 40, 457–463 (1997). https://doi.org/10.1007/BF02875413
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DOI: https://doi.org/10.1007/BF02875413