Abstract
Ribavirin treatment of cultured potato shoot tips was tested as a means of eradicating potato virus X (PVX) from two potato cultivars. Shoot tips were cultured on liquid medium containing 0, 1, 10 or 100μg/ml ribavirin. Cultures were evaluated periodically for viability, and scored for vigor on a relative growth scale. Developed plantlets were assayed for PVX by transmission tests toGomphrena globosa.
Ribavirin treatment was phytotoxic at all tested concentrations, and lethal to all cultivars treated at 100 μg/ml. Treatment also delayed plantlet development by up to 2 months at 1 and 10 μg/ml as compared with nontreated controls. PVX assays indicated that 80 and 83% of the plantlets were free of PVX following treatment with 10 μg/ml for cultivars Russet Burbank and Red McClure, respectively. Five and 6% of the plantlets developed from the 1 μg/ml treatment were PVX-free, whereas 0 and 2% of the controls were PVX-free for the same cultivars. Six to 8 months were required to develop plants from shoot-tip cultures treated with 10 μg/ml ribavirin.
Resumen
Tratamiento con Ribavirin de ápices de brotes de papa “in vitro” fue probado como un medio de erradicar al virus X de la papa (PVX) de dos cultivares. Apices de brotes fueron cultivados en medio liquido conteniendo 0, 1, 10 ó 100 μg/ml de Ribavirin. Los cultivos fueron evaluados periodicamente para determinar su viabilidad y calificados con relación a su vigor en una escala relativa de crecimiento. Las plántulas que desarrollaron fueron analizadas para PVX por pruebas de transmisión aGomphrena globosa.
El tratamiento con Ribavirin fue fitotóxico en todas las concentraciones probadas y letales a todos los cultivares a 100 μg/ml. El tratamiento también redujo el desarrollo de las plántulas hasta en 2 meses a 1 y 10 μg/ml en comparación con los testigos no tratados. Las pruebas para PVX indicaron que 80 y 83% de las plántulas estuvieron libres de PVX con el tratamiento de 10 μg/ml para los cultivares Russet Burbank y Red McClure, respectivamente. Cinco y 6% de las plántulas que desarrollaron del tratamiento con 1 μg/ml estuvieron libres de PVX, mientras que 0 y 2% de los testigos estuvieron libres para los mismo cultivares. Se necesitaron 6 a 8 meses para desarrollar plantas a partir de ápices de brotes tratados con 10 μg/ml de Ribavirin.
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Supported by the Colorado State University Experiment Station and published as Scientific Series Paper No. 2677.
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Klein, R.E., Livingston, C.H. Eradication of potato virus X from potato by ribavirin treatment of cultured potato shoot tips. American Potato Journal 59, 359–365 (1982). https://doi.org/10.1007/BF02875354
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DOI: https://doi.org/10.1007/BF02875354