A comparative research of zymograms of lactate dehydrogenase in different tissues of 25 species of vertebrates

Abstract

The distribution and characteristics of LDH isoenzymes in different tissues of 25 species of vertebrate were comparatively analysed by discontinuous polyacrylamide gel disk electrophoresis, combined with experiments on heat and urea inhibition. The result showed that in different tissues ofCtenopharyngodon idellus, Carassius, ayratus, Trichiurus haumete andMiichthys miiuy in addition to the distribution of LDH isoenzymes, composed of A and B subunits, a E4 isoenzyme band, moving very fast to the positive pole, was detected in the eye tissue ofTrichiurus haumele and a F4 isoenzyme hand, moving very slow to the positive pole, was found in liver and kidney ofCtenopharyngodon idellus. The A4 isoenzyme band was found in different tisseues ofPleuronichthys cornutus, inhabiting on the bottom of the ocean. From the amphition to the mammal, with the exception ofHyta arboreu immaculata, Chinnemys reevesii, Trionyx sinensis, Gallus gallus domesticus andCorvus macrorhynchos, which have special electrophoretic behaviours. In different tissues of the other animals distributed the five kinds of tetrameter of isoenzyme bands composed of A and B subunits. Meanwhile, the C4 isocnzyme band was detected in the testicle tissues of animals. The C4 bandof Rattus norvegicus caraco andLepus timidus located between the bands of LDH4 and LDHS and the C4 band ofErinaceus europaeus, a primitical animal, located to the farther side of LDHS and moving very slow to the positive pole. The distribution characteristics of LDH isoenzymes of different classes of vertebrates were compared and it was suggested that no parallism existed between the changes of LDH isoenzyme zymograms and phylogeny of vertebrates.