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Relationships between pathotypes and RAPDs, Gpi-allozyme patterns, mating types, and resistance to metalaxyl ofPhytophthora infestans in Canada in 1997

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Abstract

Relationships between pathotypes and other markers such as mating types, metalaxyl resistance, Gpi-allozyme and random amplified polymorphic DNA (RAPD) patterns, were investigated among 33 isolates ofPhytophthora infestans obtained from both potato and tomato across Canada in 1997. Pathogenicity testings, based on virulence against potato differentials carrying single-resistance genes and the size of lesions on leaves, revealed higher pathogenic diversity in 1997 than in the few past years, with more pathotypes and more race-complex isolates. A higher pathogenic diversity was observed within A2 (US-8) than within A1 (US-11) isolates. There was no correlation between the level of resistance to metalaxyl of an isolate and its pathogenicity. Nevertheless, when grouped into metalaxyl-sensitive (MS), -intermediate (MI), and -resistant (MR) classes, both average pathotype complexity and average lesion size were lowest for MS group. RAPD data revealed a good distinction between A1 (US-11) and A2 (US-8) mating type groups, and between isolates from British Columbia and those from other provinces. The detection in the last few years of both A1 and A2 mating types in Canada and the occurrence in 1997 of some isolates sharing different characteristics of both of these two groups suggest that sexual reproduction may have occurred locally.

Resumen

Se investigaron las relaciones entre patotipos y otros mart-adores, como tipos de apareamiento, resistencia al metalaxil, alozima Gpi y patrones de polimorfismo en el ADN amplificado aleatoriamente (RAPD), entre 33 cultivos aislados dePhytophthora infestans obtenidos de cruces de papa y tomate en Canada en 1997. Las pruebas de patogenicidad, basadas en la virulencia contra diferenciales de papa portadoras de genes simples de resistencia y el tamaño de la lesión en las hojas, revelaron una mayor diversidad patógena en 1997 que en los años previos recientes, con aislamiento de mayor cantidad de patotipos y cepas complejas. En A2 (US-8) se observó una mayor diversidad patógena que en Al (US-11). No hubo correlación entre el nivel de resistencia a metalaxil de un cultivo aislado y su patogenicidad. No obstante, cuando se agrupó por clases, de acuerdo a sensibilidad al metalaxil (SM), -intermedia (MI) y -resistente (MR), tanto el promedio de complejidad del patotipo como el tamaño promedio de la lesión fueron menores para el grupo SM. Los datos del RAPD revelaron una gran diferencia entre los grupos de apareamiento A1 (US-11) y A2 (US-8) y entre los cultivos aislados en British Columbia con los de otras provincias. La detección en años recientes de los tipos de apareamiento A1 y A2 en Canadá y la ocurrencia en 1997 de algunos aislados que comparten características diferentes de ambos grupos, sugieren que la reproducción sexual podría haber ocurrido localmente.

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Authors and Affiliations

  1. Agriculture and Agri-Food Canada, Crops and Livestock Research Centre, 440 University Avenue, CIA 7M8, Charlottetown, PEI, Canada

    F. Daayf, H. W. (Bud) Platt, G. Mahuku & R. D. Peters

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  1. F. Daayf
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  2. H. W. (Bud) Platt
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  3. G. Mahuku
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Correspondence to H. W. (Bud) Platt.

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Daayf, F., Platt, H.W.(., Mahuku, G. et al. Relationships between pathotypes and RAPDs, Gpi-allozyme patterns, mating types, and resistance to metalaxyl ofPhytophthora infestans in Canada in 1997. Am. J. Pot Res 78, 129–139 (2001). https://doi.org/10.1007/BF02874768

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