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Theoretical study of the separation mechanism of ionizable compounds in capillary electrochromatography

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Abstract

The migration mechanism of ionizable compounds in capillary electrochromatography (CEC) is more complicated than in high perfomance liquid chromatography (HPLC) due to the involvement of electrophoresis and the second chemical equilibrium. The separation mcchanism of ionizable compounds in CEC has been studied theoretically. The electrochromatographic capacity factors of ions (k’) in CEC and in the pressurized CEC are derived by phenomenologicul approach. The influence of pH, voltage, pressure onk’ is discussed. In addition, thek’ of weak acid and weak base are derivcd base on acid-base equilibrium and the influence of pH onk’ is studied theoretically.

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Authors and Affiliations

  1. National Chromatographic R&A Center, Dalian Institute of Chemical I’hysics, Chinese Academy of Sciences, 116011, Dalian, China

    Mingliang Ye, Hanfa Zou, Zhen Liu, Jun Zhu, Jianyi Ni & Yukui Zhang

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  1. Mingliang Ye
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  2. Hanfa Zou
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  3. Zhen Liu
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  4. Jun Zhu
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  5. Jianyi Ni
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  6. Yukui Zhang
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Project suppoted by the Excellent Young Scientist Award from the National Natural Science Foundation of China and the Natural Science Foundation of Lioanning Province.

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Ye, M., Zou, H., Liu, Z. et al. Theoretical study of the separation mechanism of ionizable compounds in capillary electrochromatography. Sc. China Ser. B-Chem. 42, 639–648 (1999). https://doi.org/10.1007/BF02874330

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  • DOI: https://doi.org/10.1007/BF02874330

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