Abstract
The course of the parasitic action of the mouldPenicillium purpurogenum on the mycelium of a plant strain ofAspergillus niger is described in plant and laboratory conditions of surface citric acid fermentation. During the infection, a successive destruction of the mycelium ofAspergillus niger sets in, as well as a decrease in citric acid production, in this case also act the specific inhibitory thermostabile materials, secreted by the parasitic mould into the medium. The main factor is the cell lysis, effected by a specific enzymolytic system of the constitutive type. This system is evolved by the parasitic mould in the nutritions medium, resp. into the mycelium of the infected mouldAspergillus niger and causes the lysis both of entire hyphae, and of isolated cell walls. By this lytic system, up to 85% of the dry weight of the cell walls is transferred into the solution as sugars, mainly as glucose, so that the chief component undergoing the lysis is a polymer carbohydrate. The optimum temperature of the action of the lytic system lies within 30–41°C, optimum pH is 4.2–5.5. In case of several other representatives of the genusPenicillium, no lytic systems have been established, at the same time their inability for the parasitic form of life on the mycelium ofAspergillus niger was proved.
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References
Archibald, A. R., Armstrong, J. J., Baddiley, J., Hay, J. B.:Teichoic acids and the structure of bacterial cell walls. Nature 191: 570, 1961.
Armstrong, J. J., Baddiley, J.: Buchanar, J. J., Davison, A. L., Kelemen, M. V., Neuhaus, F. C.:Teichoic acids from bacterial walls. Nature 184: 247, 1959.
Brown, A. D.:Inhibition by spermine of the action of bacterial cell-wall lytic enzyme. Biochim. biophys. Acta 44: 178, 1960.
Brown, A. D.:The peripheral structure of Gramnegative bacteria I. Cell wall protein and the action of a lytic enzyme system of a marine pseudomonad. Biochim. biophys. Acta 48: 352, 1961.
Dierick, L., Ghuysen, J. M.:Purification de la β-(1,4)-N-acetylhexosaminidase sécretée par Streptomyces albus G et active sur les parois de Micrococcus lysodeikticus. Biochim. biophys. Acta 58: 7, 1962.
Furuya, A., Ikeda, Y.:Über von Streptomyces gebildete zellmembranlösende Enzyme. II. Über das lytische Enzym von Streptomyces, Stamm No.-62. J. Agric. Chem. Soc. Japan 34: 38, 1960.
Gorlenko, M. B., Levkina, L. M., Uspenskaya, G. D., Cinnov, E. A.:Investigation of the physiology and biochemistry of some parasitic moulds. (In Russian). Scientific papers of the university of Moscow. Ser. VI, Biology, Science of soil 49, 1962.
Hais, I. M., Macek, K.:Paper chromatography. Publ. House Czech. Acad. Sci., Prague, 1959.
Horikoshi, K., Iida, S.:Lysis of fungal mycelia by bacterial enzymes. Nature 181: 917, 1958.
Horikoshi, K., Iida, S.:Effect of lytic enzyme from Bacillus circulans and chitinase from Streptomyces sp. on Aspergillus oryzae. Nature 183: 186, 1959.
Leopold, H., Fencl, Z.:Studie über die Biosynthese der Zitronensäuregärung nach dem Oberflächenverfahren. Chem. Technik 10: 507, 1958.
Leopold, J., Valtr, Z., Seichertová, O.:Characteristics of fungi of the Penicillium genus in the fermentation production of citric acid. (In Czech). Čs. mikrobiool. 3: 377, 1958.
Leopold, J., Seichertová, O.:The parasitism of the mould Penicillium purpurogenum on Aspergillus niger. I. Isolation of cell walls from the mycelium of Aspergillus niger grown by the surface method. Fol. microbiol. 12: 345, 1967.
Noel de Terra, Tatum, E. L.:The growing of colonies of Neurospora crassa. The composition of the cell wall and the morphological alterations caused by sorbose and enzymes. Science 134: 1066, 1961.
Palej, T., Ositsheva, A. G.:On the relationship of A. niger and P. luteumpurpurogenum Thom and Church. Scientific Papers on the production of citric acid by way of biochemistry. Ed. by the Food-Industries. Leningrad-Moscow 1936.
Pelzer, H., Maass, D., Weidel, W., Primosigh, J.:Die enzymatische Spaltung der Mucopeptidschicht von Escherichia-coli-zell-wänden durch ein Enzym aus dem Bacteriophagen T2 oder durch Lysozym. Vortrag. Angew. Chemie 73: 44, 1961.
Raper, K. B., Thom, Ch.:A manual of the Penicillia. Williams & Wilkins Co., Baltimore, 1949.
Salton, M. R. J., Ghuysen, J. M.:Acetylhexosamine compounds enzymically released from Micrococcus lysodeikticus cell walls III. The structure of di-and tetra-saccharides released from cell walls by lysozyme and streptomyces F 1 enzyme. Biochim. biophys. Acta 45: 355, 1960.
Sumere, C. F. van, Sumere-De Preter, C. van, Ledingham, G. A.:Cell wall-splitting enzymes of Puccinis graminis var. tritici. Can. J. Microbiol. 3: 761, 1957.
Täufel, K., Ruttloff, H.: Przyborouski, E.:Biochemische Differenzierung von Zuckergemischen mittels Presshefe II. Aufteilung bekannter Gemische. Die Nährung 4: 512, 1960.
Thom, Ch., Raper, K. B.:A manual of the Aspergilli. The Williams & Wilkins Co., Baltimore, 1945.
Wolf, J.:Microscopical technique (In Czech), Stát. zdrav. nakl., p. 281, Prague, 1954.
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Leopold, J., Seichertová, O. The parasitism of the mouldPenicillium purpurogenum onAspergillus niger . Folia Microbiol 12, 350–357 (1967). https://doi.org/10.1007/BF02873810
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DOI: https://doi.org/10.1007/BF02873810