Abstract
The deoxyribonuclease activity was investigated in cell-free filtrates of cultures of strains of different transformability. The highly transformable stable strains display a relatively low, probably optimal, deoxyribonuclease activity. Transformable but unstable strains produce a very high deoxyribonuclease activity in comparison with the aforementioned ones. In some nontransformable strains no deoxyribonuclease activity could be detected. The role of deoxyribonuclease in the competence of recipient cells s discussed.
Similar content being viewed by others
References
Ephrussi-Taylor, H.:The mechanism of deoxyribonucleic acid induced transformations. In: Recent Progr. Microbiol. Symp. 7th int. Congr. Microbiol. p. 51, Stockholm: Almquist & Wiksell, 1958.
Fox, M., Allen, M.:On the mechanism of deoxyribonucleate integration in pneumococcal transformation. Proc. nat. Acad. Sci. 52: 412, 1964.
Kohoutová, M.:Effect of monovalent cations on type specificity transformation in pneumococci. Nature: 182: 188, 1958. Čs. mikrobiool. 3: 144, 1958.
Kohoutová, M.:Mechanism of the transformation of the polysaccharide capsule in Pneumococcus. Nature 190: 1171, 1961.
Kohoutová, M.:Contribution on the biochemical investigation of external factors required for type transformation in the Pneumococcus. Fol. microbiol. 7: 33, 1962.
Kohoutová, M.:Kinetics of the potassium- and sodium-activated infection of a transforming deoxyribonucleic acid in Pneumococcus. J. gen. Microbiol. 38: 211, 1965.
Kohoutová, M.:Role of deoxyribonuclease in genetic transformation. II. RNA as an inhibitor of deoxy ribonuclease activity in pneumococcal strains. Fol. microbiol. 12: 316, 1967.
Lacks, S.:Molecular fate of DNA in genetic transformation of Pneumococcus. J. mol. Biol. 5: 119, 1962.
Ottolenghi, E., Hotchkiss, R. D.:Release of genetic transforming agent from pneumococcal structures during growth and disintegration. J. exptl. Med. 116: 491, 1962.
Ottolenghi, E.:DNA-mediated transformations between bacterial cells. Proc. Symp. Mutational Process, G. Mendel, Symp. p. 173. Academia. Publ. House Czechoslovak Acad. Sci., Praha. Acad. Press, New York and London, 1966.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kohoutová, M. Role of deoxyribonuclease in genetic transformation. Folia Microbiol 12, 311–315 (1967). https://doi.org/10.1007/BF02873805
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02873805