Abstract
A seven-step selection procedure (repeated UV irradiation, single-step application of nitrous acid, and natural selection, following each mutagenic treatment) made it possible to increase production of mithramycin byStreptomyces atroolivaceus from 40–50 μg/ml to 680–830 μg/ml,i.e. roughly by 15 to 19-fold. The UV radiation was more effective when applying lower doses, yielding about 5% survival, 2.3% survival was obtained after the treatment with nitrous acid.N-Methyl-N′-nitro-N-nitrosoguanidine applied in a buffer pH 9.0) at doses yielding more than 99% killing was less effective than the two former mutagens.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Alikhanyian S. I., Goldat S. Yu., Klepikova F. S., Mindlin S. Z.: Utilization of ethyleneimine in selection of strains producing penicillin. (In Russian)Antibiotiki,1, 33 (1957).
Bakhaeva G. P., Berlin Yu. A., Boldyreva E. F., Chuprunova O. A., Kolosov M. N., Soifer V. S., Vasilyeva T. E., Yartseva I. V.: The structure of aureolic acid (mithramycin).Tetrahedron Letters 3595 (1968).
Blumauerová M., Ismail A. A., Hošťálek Z., Callieri D. A. S., Cudlín J., Vaněk Z.: Regulation of biosynthesis of secondary metabolites. XV. Isolation and characterization of auxotrophic mutants inStreptomyces aureofaciens.Folia Microbiol. 19, 474 (1973a).
Blumauerová M., Hošťálek Z., Vaněk Z.: Mutagenesis by UV-irradiation and N-methyl-N′-nitro-N-nitrosoguanidine inStreptomyces aureofaciens. Proc. Internat. Conf. “The Bases of the Biological Effects of Ultraviolet Radiation”, Brno 1972.Stud. Biophys.36/37 311 (1973b).
Delić V., Hopwood D. A., Friend E. J.: Mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine (NTG) inStreptomyces coelicolor Mutation Res 9, 167 (1970).
Gauze G. F., Maksimova T. S., Ukholina R. S., Brazhnikova M. G., Krugliak E. B.:Act. atroolivaceus, a new mithramycin producing organism. (In Russian)Antibiotiki 12, 1059 (1967).
Goldat S. Yu.: Selection ofActinomyces aureofaciens (a producer of chlortetracycline) by means of mutagen factors. (In Russian),Trudy Inst. Mikrobiol. Akad. Nauk SSSR 10, 159 (1961).
Grundy W. E., Goldstein A. W., Rickher C. J., Hanes M. E., Warren H. B., Sylvester J. C.: Aureolic acid, a new antibiotic. I. Microbiologic studies.Antibiotics & Chemother.3, 1215 (1953).
Herold M., Bělík E., Doskočil J.: Biosynthesis of chlortetracycline without maintenance of aseptic conditions.Giorn. Microbiol.2 302 (1956).
Hopwood D. A., Sermonti G.: The genetics ofStreptomyces coelicolor.Adv. Genet.11, 273 (1962).
Hovorková N., Cudlín J., Matějů J., Blumauerová M., Vaněk Z.: Microbial glucosidation of alizarinand anthraflavin.Coll. Czech. Chem. Commun. 39, 662 (1974a).
Hovorková N., Cudlín J., Matějů J., Blumauerová M., Vaněk Z.: Microbial glucosidation of monohydroxyanthraquinones.Coll. Czech. Chem. Commun.39, in press (1974b).
Mindlin S. Z., Alikhanyian S. I.: A study on UV-induced variation ofActinomyces rimosus (oxytetracycline producer) and its selection. (In Russian)Antibiotiki 3, 18 (1958).
Rao K. V., Cullen W. P., Sobin B. A.: A new antibiotic with antitumor properties.Antibiotics & Chemother.12, 182 (1962).
Stark W. M., Knox N. G., Wilgus R. M.: Strain ofStreptomyces tenebrarius and biosynthesis of nebramycin.Folia Microbiol.16, 205 (1971).
Tessman J., Poddar K. R., Kumar S.: Identification of the altered bases in mutated single-stranded DNA. I.In vitro mutagenesis by hydroxylamine, ethyl methanesulfonate and nitrous acid.J. Mol. Biol.9, 352 (1964).
Thoma R. W.: Use of mutagens in the improvement of production strains of microorganism.Folia Microbiol.16, 197 (1971).
Vaněk Z.: Arrival of biogenetically tailored antibiotics In: M. Hejzlar, M. Semonský and S. Masák (Eds.)-Advances in Antimicrobial and Antineoplastic Chemotherapy I/2.Progress in Research and Clinical Application (Proc. VIIth Internat. Congr. Chemother., Prague 1971), p. 783, Avicenum, Prague 1973.
Vaněk Z., Tax J., Komersová I., Eckardt K.: Glycosidation of ε-pyrromycinone using the strainStreptomyces galilaeus JA 3043.Folia Microbiol.18, 524 (1973).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Blumauerová, M., Callieri, D.A.S., Stajner, K. et al. The study of variability and strain selection inStreptomyces atroolivaceus . Folia Microbiol 19, 133–138 (1974). https://doi.org/10.1007/BF02872846
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02872846