Abstract
Plasma membrane particles demonstrable by the freeze-etching technique, play, according to some authors, a role in the cell wall synthesis. On a model of yeast protoplast capable of regenerating the cell wall we studied the morphology of plasma membrane particles and the synthesis of the fibrillar cell wall component following a treatment with various enzymes and with lysolecithin. The enzymes used included proteases (trypsin, papain, pronase), polysaccharide-splitting enzymes (snail enzyme complex, mannosidase), phospholipases (A, C, D) and lipase. Upon treating living protoplasts with these substances in no case did we observe any morphologically demonstrable change in the particle structure or in their distribution in the plasma membrane. The fibrillar cell wall component was synthetized even in the presence of proteases and phospholipases. If the plasma membrane particles are assumed to represent enzyme systems synthesizing the cell wall component then in living protoplasts they are not located on the outer plasma membrane face or else are protected by some mechanism against the action of the corresponding enzymes.
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Nečas, O., Svoboda, A. Effect of proteases, phospholipases and polysaccharide-splitting enzymes on plasma membrane particles and on the synthesis of the fibrillar cell wall component in yeast protoplasts. Folia Microbiol 19, 81–87 (1974). https://doi.org/10.1007/BF02872839
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DOI: https://doi.org/10.1007/BF02872839