Skip to main content
Log in

Cultivation of the yeastCandida lipolytica on hydrocarbon

III. Oxidation and utilization of individual pure hydrocarbons

  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

The ability of the yeastCandida lipolytica 4-1 to oxidize and utilize various pure aliphatic hydrocarbons occurring in gas oil was studied. It was found that the given strain ofCandida lipolytica oxidized n-alkanes without adaptation, starting with heptane, and utilized them for growth, starting with nonane. Isoalkanes with a single methyl group in the side chain were also oxidized and utilized for growth, but less than the corresponding n-alkanes. The site of the methyl group in the isoalkane chain influences its conversion to biomass. Branched chains at both ends of the isoalkane molecule prevent its utilization for growth ofCandida lipolytica. 1-olefines are also oxidized and utilized for growth, though less than the corresponding n-paraffins. Alkylaromatic hydrocarbons are oxidized from amylbenzene up to decylbenzene, which is utilized only slightly for growth of the yeast.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Asinger, F.:Verfahren zur quantitativen oxydativen Aufspaltung von höhermolekularer Olefine. Ber. 75: 658, 1942.

    Google Scholar 

  • Bláha, K.:Preparative reactions in organic chemistry VI. (In Czech) Publ. House Czech. Acad. Sci., Prague 1961.

    Google Scholar 

  • Brooks, B. T., Kurtz, S. S., Boord, C. E., Schmerling, E.:The Chemistry of petroleum hydrocarbons. (Chinese reprint.) Vol. 1, p. 506, 1954.

    Google Scholar 

  • Buchta, E., Weidinger, H.:Synthese mit 1,4-Butylendimagnesium-bromid. Ann. 580: 109, 1953.

    CAS  Google Scholar 

  • Canin, D. L., Forziati, A. F., Rossini, F. D.:Physical properties of n-hexadecane, n-decylcyclopentane, n-decylcyclohexane, 1-hexadecane and n-decylbenzene. J. phys. Chem. 58: 440, 1954.

    Article  Google Scholar 

  • Champagnat, A., Vernet, C., Laine, B., Filosa, J.:Biosynthesis of proteinvitamin concentrate of petroleum. Nature 197: 13, 1963a.

    Article  CAS  Google Scholar 

  • Champagnat, A., Vernet, C., Laine, B., Filosa, J.:Déparaffinage microbiologique avec production de concentrates protéines-vitamines. Proc. VI. World Petroleum Congress, Frankfurt am Main, Sec. IV, paper 4—PD 10, 1963b.

  • Diuguid, L. I.:The use of amalgamated aluminium as a catalyst in the Frieden and Crafts reaction. J. Am. chem. Soc. 63: 3527, 1941.

    Article  CAS  Google Scholar 

  • Dostálek, M., Munk, V., Volfová, O., Pecka, K.:Cultivation of the yeast Candida lipolytica on hydrocarbons. I. Degradation of n-alkanes in batch fermentation of gas oil. Biotechnol. & Bioeng. 10: 33, 1968a.

    Article  Google Scholar 

  • Dostálek, M., Munk, V., Volfová, O., Fencl, Z., Pecka, K.:Cultivation of the yeast Candida lipolytica on hydrocarbons. II. One-stage continuous cultivation on gas oil. Biotechnol. & Bioeng. 10: 865, 1968b.

    Article  Google Scholar 

  • Edgar, G., Calingaert, G., Marer, R. E.:The preparation and properties of the isomeric heptanes. I. Preparation. J. Am. chem. Soc. 51: 1483, 1929.

    Article  CAS  Google Scholar 

  • Fahim, H. A., Mustafa, A.:A comparative study of the Wurtz-Fittig and the Clemmensen method for the preparation of aromatic hydrocarbons. J. chem. Soc. (London) 519, 1949.

  • Faillebin, M.:Sur la préparation de l'hexan normal. Bull. Soc. Chim. Fr. 35: 160, 1924.

    CAS  Google Scholar 

  • Grignard, V.:Les récentes acquisitions des méthodes organomagnésiennes. Bull. Soc. Chim. Fr. 39: 1285, 1926.

    CAS  Google Scholar 

  • Kazansky, B. A., Liebman, A. L., Plate, A. F., Rosengart, M. I., Tarasova, G. A.:Synthesis and physical characteristics of some 1-alkanes. (In Russian) Zhur. Obshch. Chim. 17: 1503, 1947.

    Google Scholar 

  • Krafft, F.:Zur Darstellung höherer Olefine, insbesondere über Dodecylen C 12 H 24,Tetradecylen C 14 H 28,Ceten oder Hexadecylen C 16 H 32 und Octadecylen C 18H36. Ber. 16: 3018, 1883.

    CAS  Google Scholar 

  • Landa, S., Kejvan, A.:Synthèse du 2,11-diméthyldodécane et du 2,19-diméthyleicosane. Coll. Czech. chem. Commun. 3: 367, 1931.

    CAS  Google Scholar 

  • Landa, S., Mostecký, J.:Preparation of pure hydrocarbons by hydrogenation on tungsten disulphide. I. Hydrogenation of alcohols, ketones, esters and acid anhydrides. Coll. Czech. chem. Commun. 20: 430, 1955.

    CAS  Google Scholar 

  • Landa, S., Weisser, O.:Preparation of hydrocarbons by hydrogenation on molybdenum disulphide. I. Hydrogenation of alcohols, ketones, acids and esters. (In Czech) Chem. Listy 50: 569, 1956.

    CAS  Google Scholar 

  • Landa, S., Weisser, O.:Preparation of hydrocarbons by hydrogenation on molybdenum disulphide. I. Hydrogenation of alcohols, ketones, acids and esters. (In Russian) Coll. Czech. chem. Commun. 22: 93, 1957a.

    Google Scholar 

  • Landa, S., Weisser, O., Mostecký, J.:Über die Eigenschaften von Sulfid-Katalysatoren. IV. Zum Mechanismus der Hydrierung sauerstoffhaltiger Verbindungen. Coll. Czech. Chem. Commun. 22: 1006, 1957b.

    CAS  Google Scholar 

  • Landa, S., Weisser, O., Mostecký, J.:Über die Eigenschaften sulfidischer Katalysatoren. VI. Die dehydratierende und dehydrierende Eigenschaften von Molybdän-und Wolframsulfid. Coll. Czech. chem. Commun. 24: 1036, 1959.

    CAS  Google Scholar 

  • Lavina, R. Y., Shusherina, N. P.:Methods of synthesis of paraffin hydrocarbons (alkanes). (In Russian) Usp. chim. 24: 181, 1955.

    Google Scholar 

  • Lukeš, R., Bláha, K.:Preparation of Grignard reagents derived from ω ω-dibromo-n-olkanes. (In Czech) Chem. Listy 46: 683, 1952.

    Google Scholar 

  • Ma, T. S., Zuazaga, G.:Microkjeldahl determination of nitrogen. A new indicator and an improved rapid method. Ind. Eng. Chem. Annual 14: 280, 1942.

    Article  CAS  Google Scholar 

  • Markham, N.:A stream distillation apparatus suitable for micro-kjeldahl analysis. Biochem. J. 36: 790, 1942.

    PubMed  CAS  Google Scholar 

  • Obolentsev, R. D.:Physical constants of hydrocarbons of liquid fuels and oils. (In Russian) Gostoptekhizdat, Moscow 1953.

    Google Scholar 

  • Petrov, A. A., Serbienko, S. R., Nechitailov, N. A., Tsedilina, A. L.:Synthesis and characteristic of monomethyl-substituted alkanes of the composition C 12 −C 16. (In Russian) Izv. A. N. SSSR, Otd. Chim. Nauk, 1091, 1959.

  • Suida, H., Drohowzal, F.:Ceten=Hexadecen=(1). Ber. 75: 991, 1942.

    Google Scholar 

  • Wooster, C. B.:Organo-alkali compounds. Chem. Rev. 11: 1, 1932.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

Dedicated to Academician Ivan Málek on the occasion of his 60th birthday

Rights and permissions

Reprints and permissions

About this article

Cite this article

Munk, V., Volfová, O., Dostálek, M. et al. Cultivation of the yeastCandida lipolytica on hydrocarbon. Folia Microbiol 14, 334–344 (1969). https://doi.org/10.1007/BF02872701

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02872701

Keywords

Navigation