Abstract
A new method permitting the study of scars on intact cells of different species of yeasts by means of secondary primulin fluorescence is described. The existence of two types of scars in budding yeasts was confirmed and their morphology was described in intact cells. In fission yeasts a number of division scars was found in individual cells and changes occurring in the lateral walls as a result of cytokinetic process were observed. In yeasts reproducing by bipolar budding, a new type of scar—the multiple scar—with an important morphogenetic function was discovered. The possibilities and prospects of the new method are discussed.
Abstract
ОписЫвается нояій метод изучения шрамов на нена рушеннЫх клетках различнЫх видов дрожжей на основании вторичной флуоресценции примулина. БЫло подтверждено наличие у почкующихся дрожжей двух видов шрамов. ОписЫвается морфология шрамов ненарушеннЫх клеток. У делящихся дрожжей бЫло обнаружено большое количество шрамов от деления (division scars) на отделънЫх клетках; наблюдалисъ изменения боковЫх ктенок, развивающиеся в резулътате цитокинетического процесса. У дрожжей с бинолярнЫм способом бегетативнобо размножения. бЫл обнаружен новЫй тип шрамов—множественнЫе шрамЫ (multiple scars), связаннЫе с важной мовфогенетической функцией.—Обсуждаются возможности и перспективЫ нового метода.
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Streiblová, E., Beran, K. Types of multiplication scars in yeasts, demonstrated by fluorescence microscopy. Folia Microbiol 8, 221–227 (1963). https://doi.org/10.1007/BF02872585
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DOI: https://doi.org/10.1007/BF02872585