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Conditions of the formation of pyrogenic substances by delayed hypersensitive cells in contact with antigenin vitro

Условия образования нирогенных веществ клетками с ноздней гиперсензитивностью нри соприкосновении с антигеном in vitro

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Summary

  1. (1)

    In a number of systematic experiments the biological conditions were determined in detail of the formation of pyrogenic substances in the reaction of hypersensitive cells with the specific antigenin vitro: the role of the quantitative relationship of cells and antigen, of temperature and incubation period, of cell viability, etc. A part of the experiments was performed with131I labelled antigen.

  2. (2)

    It has been demonstrated that pyrogenic substances are also formed on mixing cell-free extracts of hypersensitive cells with specific antigen, and the conditions of this reaction are defined.

  3. (3)

    In various experimental conditions a significant conformity was found between the formation of pyrogenic substances inin vitro experiments and the possibility of passive transfer of systemic delayed type hypersensitivity.

  4. (4)

    After testing pyrogenic substances and after provoking the systemic hypersensitivity reaction in passively sensitized animals, an increased susceptibility to the pyrogenic effect of other substances was observed.

  5. (5)

    The experimental results presented are discussed from the point of view of our previous papers and with regard to contemporary knowledge on the mechanism and significance of delayed type hypersensitivity.

Abstract

  1. (1)

    В ряде систематических оплтов дыли подробно определены биологические условия возникновения пирогенных веществ при реакции гиперсензитивных клеток со специфическим антигеном in vitro, влияние количества клеток и антигена, температуры и продояжительности инкудации, жизнеспосодности клеток и т. п.

  2. (2)

    Было доказано, что пирогенные вещества одразуются и в смеси бесклеточных зкстрактов гиперсензитивных клеток со специфическим антигеном, и дыли изучены условия зтой реакции.

  3. (3)

    При различных условиях опыта наблюдалась значителяная корреляция между образованием пирогенных веществ в опытах in vitro и возможностью пассивной передачи позднего типа гиперсензитивности.

  4. (4)

    Было найдено, что у пассивно сенсибилизированных дивотных после тестирования пирогенных веществ и после индукции реакции гиперсензитивности повышается чувствительность к пирогенному действию других веществ.

  5. (5)

    Указанные результаты опытов обсуждаются с точки зрения данных наших предшествующих работ и современных сведений относительно механизма и значения гиперсензитивности позднего типа.

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Authors and Affiliations

  1. Institute of Sera and Vaccines, Prague 10

    J. Johanovský

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  1. J. Johanovský
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Johanovský, J. Conditions of the formation of pyrogenic substances by delayed hypersensitive cells in contact with antigenin vitro . Folia Microbiol 6, 213–224 (1961). https://doi.org/10.1007/BF02872525

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