Abstract
A refuge of conventional potato plants adjacent to Colorado potato beetle (CPB),Leptinotarsa decemlineata (Say)-resistant,Bt-potato (transgenic) plants may reduce opportunity for the development of tolerance to the resistant plants. The refuge strategy was developed on the basis of data available for CPB recruitment in conventional potato fields. This study was undertaken to provide information on CPB recruitment inBt-transgenic potato fields. A marking experiment was conducted over the 2000 and 2001 crop seasons to determine the relative contributions of beetle populations from fields 10 m, 175 to 300 m, and 1200 to 1280 m distant to the pattern of CPB recruitment (immigration) in aBt-potato field. Season-long CPB recruitment in theBt-potato field decreased with source distance in a manner similar to that previously reported in conventional potato fields. Although marked beetles from the 10-m field plot contributed more to the recruitment than the more distant fields, they contributed only 3.3% and 6.6% of the total beetle sightings in theBt-potato field. Therefore, results suggest that a larger acreage of conventional potato fields at some distance from the resistant crop could replace the adjacent designated refuge. Seasonally, beetles from the overwintering sites provided the first recruits to the resistant field. As their contribution declined, the overwintered beetles from the refuge were recruited until the beginning of the summer population. These results suggest that locating the resistant fields close to active overwintering sites will improve the probability that the refuge strategy will be effective by ensuring the presence of recruits at the very beginning of the crop season. The substantially lower recruitment level obtained for summer than for overwintered CPB in resistant and conventional potato fields highlights the need to reconsider the applicability of the refuge strategy for the summer population.
Resumen
Papas convencionales sembradas como refugio adyacentes a papas-BT (transgénicas) resistentes al escarabajo colorado de la papa (CPB)Leptinotarsa decemlineata (Say), pueden reducir la probabilidad de desarrollar tolerancia a las plantas resistentes. La estrategia de refugio fue desarrollada sobre la base de datos disponibles de inmigración de CPB en campos convencionales de papa. Este estudio se hizo para proporcionar información sobre la inmigración de CPB en campos de papaBT-transgénicas. Se llevó a cabo un experimento marcado en las campañas agrícolas 2000 y 2001 para determinar la contribución de las poblaciones del escarabajo de campos de 10m, 175–300m y 1200–1280m de distancia al patrón de inmigración del CPB en un campo de papa-Bt. La inmigración del CPB en el campo de papa-Bt durante todo el periodo de cultivo disminuyó de acuerdo a la fuente-distancia de manera similar al reportado previamente en campos convencionales de papa. Aunque los escarabajos marcados de la parcela de 10 m contribuyeron más a la inmigración que los de campos más distantes, sólo contribuyeron con el 3.3% y 6.6% del total de escarabajos vistos en el campo de papa-Bt. Por lo tanto, los resultados sugieren que áreas grandes de campos de papa convencionales sembradas a cierta distancia del cultivo resistente, podrían reemplazar al refugio señalado adyacente. Estacionalmente, los escarabajos provenientes de lugares donde inviernan proporcionaron los primeros inmigrantes a los campos con papa resistentes. A medida que la contribución declinó, los escarabajos invernados de los refugios fueron contabilizados hasta el inicio de la población de verano. Estos resultados sugieren que la ubicación de campos resistentes cerca de los lugares activos de invernación del escarabajo, mejorará la probabilidad de que la estrategia del refugio sea efectiva asegurando la presencia de inmigrantes muy al inicio de la estación de cultivo. El mas bajo nivel de inmigración obtenido para el verano que el de proveniente de PCB invernados para los campos con cultivos resistentes y convencionales de papa, destaca la necesidad de reconsiderar la aplicabilidad de la estrategia de refugio para la población de verano.
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Abbreviations
- CPB:
-
Colorado potato beetle
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Boiteau, G. Recruitment of adult Colorado potato beetles inBt-transgenic potato fields. Am. J. Pot Res 82, 379–387 (2005). https://doi.org/10.1007/BF02871968
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DOI: https://doi.org/10.1007/BF02871968