Summary
The effect of different stimuli on the expression of the low-affinity receptor for the Fc fragment of IgE (FcɛR2/CD23) on peripheral blood B cells from patients with chronic lymphocytic leukemia (CLL) was investigated. CLL B cells cultured for 3 days in medium alone showed a progressive decrease of the FcɛR2/CD23 expression, while the addition to the cell cultures of IgE or interleukin-4 had a slackening effect on the decrease of the FcɛR2/CD23. In contrast, in the presence of interferon-γ the proportion of FcɛR2/CD23+ cells was more rapidly reduced compared to CLL B cells cultured in medium alone. Stimulation of CLL B cells withStaphylococcus aureus Cowan I (SAC) bacteria, which are able to enhance the expression of FcɛR2/CD23 on normal B cells, induced a rapid loss of the FcɛR2/CD23 from CLL B cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bonnefoy J. Y., Aubry J. P., Peronne C., Wijdens J., Banchereau J.: Production and characterization of a monoclonal antibody specific for the human lymphocyte low affinity receptor for IgE: CD23 is a low affinity receptor for IgE — J. Immunol.138, 2970, 1987.
Capron M., Capron A., Dessaint P., Torpier G., Johansson S. G. O., Prin L.: Fcɛ receptors for IgE on human and rat eosinophils — J. Immunol.126, 2087, 1981.
De France T., Aubry J. P., Rousset F., Vanbervliet B., Bonnefoy J. Y., Arai N., Takebe Y., Yokota T., Lee F., Arai K., de Vries J. E., Banchereau J.: Human recombinant interleukin 4 induces Fcɛ receptors (CD23) on normal human lymphocytes — J. exp. Med.165, 1459, 1987.
Delespesse G., Sarfati M., Rubio-Trujillo M.:In vitro production of IgE-binding factors by human mononuclear cells — Immunology60, 103, 1987.
Delespesse G., Sarfati M., Rubio-Trujillo M., Wolowiec T.: IgE receptors on human lymphocytes. II. Detection of cells bearing IgE receptors in unstimulated mononuclear cells by means of a monoclonal antibody — Europ. J. Immunol.16, 815, 1986.
Freedman A. S., Boyd A. W., Bieber F. R., Daley J., Rosen K., Horowitz J. C., Levy D. N., Nadler L. M.: Normal cellular counterparts of B cell chronic lymphocytic leukemia — Blood70, 418, 1987.
Galizzi J. P., Cabrillat H., Rousset F., Menetrier C., de Vries J. E., Banchereau J.: Interferon-γ and prostaglandin E2 inhibit interleukin 4-induced expression of FcɛR2/CD23 on B lymphocytes through different mechanisms without altering binding of IL-4 to its receptor — 1988. (In press).
Gonzales-Molina A., Spiegelberg H. L.: A subpopulation of normal human peripheral B lymphocytes that bind IgE — J. clin. Invest.59, 616, 1977.
Gordon J., Rowe M., Walker L., Guy G.: Ligation of the CD23, p45 (BLAST-2, EBVCS) antigen triggers the cell-cycle progression of activated B lymphocytes — Europ. J. Immunol.16, 1075, 1986.
Gordon J., Webb A. J., Guy G. R., Walker L.: Triggering of B lymphocytes through CD23: epitope mapping and studies using antibody derivatives indicate an allosteric mechanism of signalling — Immunology60, 517, 1987.
Grillot-Courvalin C., Hivroz C., Vallee A., Banchereau J., Brouet J. C.: Regulation of CD23 antigen expression on normal and malignant B cells by IL-2: comparison with IL-4. In: VI Int. Lymphokine Workshop. Evian, October 23–27, 1988; p. 308. (Abstract).
Joseph M., Capron A., Ameisen J.-C., Capron M., Vorng H., Pancre V., Klusnierz J. P., Auriault C.: The receptor for IgE on blood platelets — Europ. J. Immunol.16, 306, 1986.
Kikutani H., Suemura M., Owaki H., Nakamura H., Sato R., Yamasaki K., Barsumian E. L., Hardy R. R., Kishimoto T.: Fcɛ receptor, a specific differentiation marker transiently expressed on mature B cells before isotype switching — J. exp. Med.164, 1455, 1986.
Kumagai K., Abo T., Sekizawa T., Sasaki M.: Studies of surface immunoglobulin on human B lymphocytes. I. Dissociation of cell bound immunoglobulin with acid pH or at 37°C — J. Immunol.115, 982, 1975.
Moller P., Moldenhauer G., Momburg F., Lammler B., Eberlein-Gonska M., Kiesel S., Dorken B.: Mediastinal lymphoma of clear cell type is a tumor corresponding to terminal steps of B cell differentiation — Blood69, 1087, 1987.
Pène J., Rousset F., Brière F., Chrétien I., Bonnefoy J. Y., Spits H., Yokota T., Arai N., Arai K., Banchereau J., de Vries J. E.: IgE production by normal human lymphocytes is induced by IL-4 and suppressed by interferon α and prostaglandin E2 — Proc. nat. Acad. Sci. (Wash.) 1988. (In press).
Pène J., Rousset F., Brière F., Chrétien I., Wideman J., Bonnefoy J. Y., de Vries J. E.: Interleukin 5 enhances interleukin 4-induced IgE production by normal human B cells. The role of soluble CD23 antigen — Europ. J. Immunol.18, 929, 1988.
Romagnani S., Damiani G., Giudizi M. G., Biagiotti R., Almerigogna F., Del Prete G. F., Maggi E., Bargellesi A., Ricci M.:In vitro production of IgE by human peripheral blood mononuclear cells. III. Demonstration of a circulating IgE-bearing cell involved in the spontaneous IgE biosynthesis — Clin. exp. Immunol.49, 176, 1982.
Romagnani S., Giudizi M. G., Almerigogna F., Biagiotti R., Bellesi G., Bernardi F., Ricci M.: Protein A reactivity of IgM- and IgD-bearing lymphocytes from some patients with chronic lymphocytic leukemia — Clin. Immunol. Immunopathol.19, 139, 1981.
Romagnani S., Giudizi M. G., Almerigogna F., Ricci M.: Interaction of staphylococcal protein A with membrane component of IgM- and/or IgD-bearing lymphocytes from human tonsils — J. Immunol.124, 1620, 1980.
Rowe M., Hildreth J. E., Rickinson A. B., Epstein M. A.: Monoclonal antibodies to Epstein-Barr virus-induced transformation-associated cell surface antigens: binding patterns and effect upon virus-specific T-cell cytotoxicity — Int. J. Cancer29, 373, 1972.
Sarfati M., Bron D., Lagneaux L., Fonteyn C., Frost H., Delespesse G.: Elevation of IgE-binding factors in serum of patients with B cell-derived chronic lymphocytic leukemia — Blood71, 94, 1988.
Spiegelberg H. L.: Structure and function of Fc receptors for IgE on lymphocytes, monocytes and macrophages — Advanc. Immunol.35, 61, 1984.
Spiegelberg H., Boltz G., Nitulescu J., Plummer J., Melewica F.: Characterization of IgE receptors on monocytes and macrophages — Fed. Proc.42, 126, 1983.
Suemura M., Kikutani H., Barsumian E. L., Hattori Y., Kishimoto S., Sato R., MacDee A., Nakamura H., Owaki H., Hardy R. R., Kishimoto T.: Monoclonal anti-Fcɛ receptor antibodies with different specificities and studies on expression of Fcɛ receptors on human B and T cells — J. Immunol.137, 1214, 1986.
Swendeman S., Thorley-Lawson D. A.: The activation antigen BLAST-2, when shed, is an autocrine BCGF for normal and transformed B cells — EMBO J.6, 1637, 1987.
Thorley-Lawson D. A., Mann K. P.: Early events in Epstein-Barr virus infection provide a model for B cell activation — J. exp. Med.162, 45, 1985.
Thorley-Lawson D. A., Nadler L. M., Bhan A. K., Scooley R. T.: Blast-2 (EBVCS), an early cell surface marker of human B cell activation, is superinduced by Epstein-Barr virus — J. Immunol.134, 3007, 1985.
Walker L., Guy G., Brown G., Rowe M., Milner A. E., Gordon J.: Control of human B-lymphocytes replication. I. Characterization of novel activation states that precede the entry of Go B cells into cycle — Immunology58, 583, 1986.
Yokota T., Otsuka T., Mosmann T., Banchereau J., De France T., Blanchard D., de Vries J., Lee F., Arai K.: Isolation and characterization of a human interleukin cDNA clone, homologous to mouse BSF-1, which expresses B cell and T cell stimulating activities — Proc. nat. Acad. Sci. (Wash.)83, 5894, 1986.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Almerigogna, F., Biagiotti, R., Giudizi, M.G. et al. The FcɛR2/CD23 antigen: A hallmark of chronic lymphocytic leukemia B cells. Res. Clin. Lab. 19, 129–138 (1989). https://doi.org/10.1007/BF02871801
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02871801